Published March 21, 2022 | Version v1
Journal article Open

MICROBIOLOGICAL STATUS OF THE MAMMARY GLAND IN LACTATING SHEEPS

Authors/Creators

  • 1. University of Forestry, Faculty of Veterinary medicine, Sofia, Bulgaria
  • 2. University of Forestry, Faculty of Veterinary medicine, Sofia, Bulgari

Contributors

  • 1. University of Forestry, Sofia, Bulgaria

Description

The aim of the present study was to determine the microbiological status of the mammary gland in lactating sheeps of the Lacon breed. For this purpose, milk samples from purebred animals, raisеd in sheep farm located in central region of Bulgaria, were collected. The results show that in 46.6% of the tested milk samples from the farm, were not isolated any pathogenic microorganisms. From the positive samples, S. xylosus was most often found, in most cases in combination with the non-pathogenic streptococcus L. lactis ssp. lactis. Only in one of the samples a combination of two staphylococcal species was found – S. epidermidis and S. xylosus, with the presence of L. lactis ssp. lactis. The number of samples with isolated bacteria with pathogenic potential was 16 (53.3%). Only in seven of them (23.3% of all examined) the amount of detected staphylococci is over 104 CFU/ml. No Gram-negative bacteria were isolated from any of the milk samples. The sensitivity of the isolated bacteria from milk samples to antibiotics from different groups is significant. Resistance has only been established to Colistin, Kanamycin, Amikacin and Gentamicin. The mean values of somatic cells in infected and uninfected halves were 1361058 ± 361307.34 and 171000 ± 36586.04, respectively.

Files

vol-7-1_2022-10-17.pdf

Files (645.7 kB)

Name Size
md5:06e9510ab6e6a84c966ec97887118b2d
645.7 kB Preview Download

Additional details

Related works

Is documented by
Journal article: https://scij-tmvm.com/vol./vol.7/1/vol-7-1_2022-10-17.pdf (URL)

References

  • Ahmad Z., S. Agarwal, A. S. Narwal, V. K. Deshwal. (2013). Comparative study of antibiotics re-sistance of coagulase negative staphylococci. Developmental Microbiology and Molecular Biology, 4, 29–33
  • Ariznabarreta A., Gonzalo, C., San Primitivo, F. (2002). Microbiological quality and somatic cell count of ewe milk with special reference to staphylococci. J. Dairy Sci. 85, 1370–1375
  • Bauer A. W., W. M. Kirby, J. C. Cherris, M. Truck. (1966). Antibiotic susceptibility testing by a standardized single disk method. The Am. J. of Clin. Pathol. 45 (4), 493–496.
  • Bergonier D., X. Berthelot. (2003). New advances in epizootiology and control of ewe mastitis. Livest. Prod. Sci. 79, 1–16.
  • Boutinaud M., Jammes, H. (2002). Potential uses of milk epithelial cells: a review. Reprod. Nutr. Dev. 42, 133–147
  • Brugère-Picoux J. (2008): Ovine listeriosis. Small Ruminant Research, 76, 12–20
  • Contreras A., D. Sierra, J.C. Corrales, J.C. Marco, M.J. Paape, C. Gonzalo. (2007). Mastitis in small ruminants. Small Ruminant Research 68, 145–153.
  • Fotou K. A.Tzora, C. H. Voidarou, A. Alexopoulos, E. Plessas, I. Avgeris, E. Bezirtzoglou, K. Akrida-Demertzi, P. G. Demertzis. (2011). Isolation of microbial pathogens of subclinical mastitis from raw sheep's milk of Epirus (Greece) and their role in its hygiene. Anaerobe, 315–319.
  • Gelasakis A.I., V.S. Mavrogianni, I.G. Petridis, N.G.C. Vasileiou, G.C. Fthenakis. (2015). Mastitis in sheep – The last 10 years and the future of research. Small Ruminant Research 88, 119–125
  • Holt J. G., N. R. Krieg, P. H. A. Sneart, J. T. Staley, S. T. (1994). Williams. Bergey's manual of determinative bacteriology, Ninth edition. Williams & Wilkins, 209–210, 538–562
  • Hristov K., T. Popova, R. Pepovich and B. Nikolov. (2016). Characterization of Microbial Causa-tive Agents of Subclinical Mastitis in Goats in Bulgaria. Int. J. Curr. Microbiol. App. Sci. 5 (8), 316–323.
  • Juste R.A., E. Minguijón, J. Arranz, M. Fuertes, I. Beltrán de Heredia. (2009). Lamb mortality in an outbreak of Yersinia pseudotuberculosis mastitis as a collateral effect of colostrums feeding for Lentivirus-control. Small Ruminant Research 86, 46–51.
  • Leitner G., U. Merin, Y. Lavi, A. Egber, N. (2007). Silanikove Aetiology of intramammary infection and its effect on milk composition in goat flocks J. Dairy Research 74, 186–193.
  • Marogna G., S. Rolesu, S. Lollai, S. Tola, G. Leori. (2010): Clinical findings in sheep farms affected by recurrent bacterial mastitis. Small Ruminant Research, 88, 119–125.
  • Martins K. B., P. Y. Faccioli, M. F. Bonesso, S. Fernandes, A. A. Oliveira, A. Dantas, L. F. Zafalon, M. de Lourdes, R. S. Cunha. (2016). Characteristics of resistance and virulence factors in different species of coagulase-negative staphylococci isolated from milk of healthy sheep and animals with subclinical mastitis. J. Dairy Sci., 100, 2184–2195
  • Mavrogianni V.S., P.I. Menzies, I.A. Fragkou, G.C. Fthenakis. (2011) Principles of mastitis treat-ment in sheep and goats. Vet. Clin. North Am. Food Anim. Pract., 27, 115–120.
  • Nebbia P., P. Robino, S. Zoppi, D. De Meneghi. (2006). Detection and excretion pattern of Myco-bacterium avium subspecies paratuberculosis in milk of asymptomatic sheep and goats by Nested-PCR. Small Ruminant Research, 66, 116–120.
  • Queiroga MC. (2017). Prevalence and aetiology of sheep mastitis in Alentejo region of Portugal. Small Ruminant Research, 153, 123–130.
  • Rodrigues M. X., S. F. Lima, C. H. S. G. Canniatti-Brazaca, R. C. Bicalho. (2016). The Lactococcus genus as a potential emerging mastitis pathogen group: A report on an outbreak investigation. Jour-nal of Dairy Science, 99, 12, 9864–9874.
  • Saratsis P., L. Leontides, A. Tzora, C. Alexopoulos, G. C. Fthenakis. (1998). Incidence risk and aetiology of mammary abnormalities in dry ewes in 10 flocks in southern Greece. Prev Vet Med., 37(1–4), 173–83.
  • Spanu C., Y. M. Berger, D. L. Thomas, P. L. Ruegg. (2011). Impact of intramammary antimicrobial dry treatment and teat sanitation on somatic cell count and intramammary infection in dairy ewes. Small Ruminant Research, 97, 139–145.
  • Sylejmani R., A. Robaj, A. Hamidi. (2015). Antimicrobial susceptibility of staphylococci isolated from clinical mastitis in dairy cows in two regions of kosovo. Albanian j. agric. sci., 14(4), 404–407.
  • Thomson K, M. Rantala, M. Hautala, S. Pyӧrälä, L. Kaartinen. (2008). Cross-sectional prospec-tivesurvey to study indication–based usage of antimicrobials in animals: Results of use incattle. Veterinary Research, 15.
  • Tvarožková K., V. Tančin, M. Uhrinčať, L. Hleba, L. Mačuhová. (2020). Mastitis pathogens and somatic cell count in ewes milk. Potravinarstvo Slovak Journal of Food Sciences, 14, 164–169.
  • Vasileiou N.G.C., P.J. Cripps, K.S. Ioannidi, D.C. Chatzopoulos, D.A. Gougoulis, S. Sarrou, D.C. Orfanou, A.P. Politis, T. Calvo Gonzalez-Valerio, S. Argyros, V.S. Mavrogianni, E. Petinaki, G.C. Fthenakis. (2018). Extensive countrywide field investigation of subclinical mastitis in sheep in Greece. J. Dairy Sci. 101, 7297–7310.