Published March 18, 2022 | Version v1
Journal article Open

Tissue-specific downregulation of fatty acid synthase sup-presses intestinal adenoma formation via coordinated repro-graming of transcriptome and metabolism in the mouse model of Apc-driven colorectal cancer

Description

Figure S1: Characterization of transgenic mouse models; Figure S2: Triglyceride levels in tissue and plasma from transgenic mice; Figure S3: Heterozygous and homozygous deletion of FASN in Apc/Cre mice is associated with an increase in citrate synthase expression; Table S1A: Samples for RNA-Seq analysis and RNA-Seq coverage summary; Table S1B: Complete report for RNA-Seq analysis; Table S1C: Transcriptome changes in (1) FASND/-/Apc/Cre vs Apc/Cre mice and (2) FASND/D/Apc/Cre vs Apc/Cre mice; Table S1D: Gene set enrichment analysis for (1) Apc/Cre vs FASN+/D/Apc/Cre (negative), (2) Apc/Cre vs FASN+/D/Apc/Cre (positive), (3) Apc/Cre vs FASND/D/Apc/Cre mice (negative), and (4) Apc/Cre vs FASND/D/Apc/Cre mice (positive); Table S2: Lipid analysis for (A) Diglycerides (DAGs) in mouse adenomas, (B) Free fatty acids (FFAs) in plasma, (C) Free fatty acids (FFAs) in mouse adenomas, and (D) Sphingolipids in mouse adenomas; Table S3: RPPA analysis of metabolic enzymes. Uncut gels.

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Uncut gels are provided in 2022_03_22_notebook_65414.4.pdf

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