Minimal dataset to test multiplexed DNA imaging (Hi-M) software pipelines

This is a dataset of nuclei (DAPI), and 3 multiplexed DNA imaging cycles to test and validate processing software packages, such as pyHiM (https://github.com/marcnol/pyHiM). This dataset was acquired in a nc14 Drosophila embryo.

File contents:

scan_001_RT27_001_ROI_converted_decon_ch00.tif     barcode 27, fiducial    
scan_001_RT27_001_ROI_converted_decon_ch01.tif     barcode 27
scan_001_RT29_001_ROI_converted_decon_ch00.tif     barcode 29, fiducial          
scan_001_RT29_001_ROI_converted_decon_ch01.tif     barcode 29     
scan_001_RT37_001_ROI_converted_decon_ch00.tif     barcode 37, fiducial             
scan_001_RT37_001_ROI_converted_decon_ch01.tif     barcode 37       
scan_006_DAPI_001_ROI_converted_decon_ch00.tif     DAPI     
scan_006_DAPI_001_ROI_converted_decon_ch01.tif     DAPI, fiducial        
scan_006_DAPI_001_ROI_converted_decon_ch02.tif     RNA

To test this dataset please refer to pyHiM documentation page.