Published February 28, 2022
| Version v1
Journal article
Open
Molecular diagnosis of SARS-CoV-2: A validation of saliva samples
Authors/Creators
- 1. Laboratory of Molecular Diagnostics, Federal University of Viçosa, campus Rio Paranaíba. Road MG 230 Km 7, LAE Building, room 237, Zip code 38810-000, Rio Paranaíba – MG, Brazil.
- 2. Center of Biotechnology, University of Mogi das Cruzes, Av. Dr. Cândido X. de Almeida e Souza, 200 - Centro Cívico, Mogi das Cruzes, SP, 08780-911, Brazil.
Description
Nasopharyngeal swabs are the most used in sample collecting for covid-19 tests in SARS-CoV-2 molecular diagnosis. However, this sampling method presents some disadvantages, since, in addition to being dependent on imported materials, it is invasive, causes discomfort in patients, and presents the risk of contamination for the medical collection team. This study aimed at validating saliva samples to obtain viral RNA to be used in the molecular diagnostic test for SARS-CoV-2 using the RT-qPCR technique. Results presented 93.44% concordance in comparison to nasopharyngeal swabs sampling. Therefore, saliva samples used in SARS-CoV-2 RT-qPCR detection tests presented consistent results.