Published October 31, 2020 | Version v1
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An investigation of traditional uses and anti-inflammatory property of Clematis buchananiana De Candolle and Tupistra nutans Wall. ex Lindl.: Native ethnomedicinal plants from Sikkim, India

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  • 1. Sikkim University

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In the traditional medicinal system of Sikkim Clematis buchananiana and Tupistra nutans is used extensively to treat various ailments, however, they have not been validated for their anti-inflammatory property by in-vitro method. Therefore, the present study was carried out to investigate specific ethnomedicinal uses, in-vitro anti-inflammatory property, and phytochemical constituents of Clematis buchananiana and Tupistra nutans. The ethnomedicinal usage was studied by calculating the value for fidelity level, use-value, and informant consensus factor. Stabilization of human red blood cell membrane and protein denaturation method was used to study anti-inflammatory property. The phytochemicals were analysed by the methods described elsewhere.
Clematis buchananiana was found to be used more frequently for sinusitis, headache, cold and Tupistra nutans for high blood pressure, diabetes and stomach-ache. Both C. buchananiana and T. nutans was found to inhibit the HRBC membrane and protein denaturation effectively in a dose-dependent manner. However, inhibition of haemolysis and protein denaturation by C. buchananiana was found to be higher than T. nutans at all doses. The phytochemical screening revealed the presence of anti-inflammatory metabolites such as flavonoids and phenolics in both the plants. The results provide evidence for the anti-inflammatory property of C. buchananiana and T. nutans.

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Journal article: 0975-1068 (ISSN)

References

  • Vane J R & Botting R M, New insights into the mode of action of anti-inflammatory drugs, Inflamm Res, 44 (1) (1995) 1-10.
  • Smith R J, Sabin C, Gilchrest H, et al., Effect of antiinflammatory drugs on lysosomes and lysosomal enzymes from rat liver, Biochem Pharmacol, 25 (19) (1976) 2171- 2177.
  • Vadivu R & Lakshmi K S, In vitro and in vivo antiinflammatory activity of leaves of Symplocos cochinchinensis (Lour) Moore ssp Laurina, Bangladesh J Pharmacol, 3 (2) (2008) 121-124.
  • Opie E L, On the relation of necrosis and inflammation to denaturation of proteins, J Exp Med, 115 (3) (1962) 597-608
  • Umapathy E, Ndebia E J, Meeme A, et al., An experimental evaluation of Albuca setosa aqueous extract on membrane stabilization, protein denaturation and white blood cell migration during acute inflammation, J Med Plant Res, 4 (9) (2010) 789–795.
  • Ostensen M E & Skomsvoll J F, Anti-inflammatory pharmacotherapy during pregnancy, Expert Opin Pharmacother, 5 (3) (2004)571-580.
  • Henry D A, Side-effects of non-steroidal anti-inflammatory drugs, Bailliere's Clin Rheumatol, 2 (2) (1988) 425-454.
  • Panda A K, Medicinal plants of Sikkim in Ayurvedic practice, (2007). Available at http://www.sikkimforest. gov.in/docs/Ayurvedhic%20Medicines.pdf
  • Idrisi M S, Badola H K & Singh R, Indigenous knowledge and medicinal use of plants by local communities in Rangit Valley, South Sikkim, India, NeBIO, 1 (2) (2010) 34-45.
  • Chaudhuri A N, Karmakar S, Roy D, et al., Antiinflammatory activity of Indian black tea (Sikkim variety), Pharmacol Res, 51 (2) (2005) 169-175.
  • Lingadurai S, Nath L K, Kar P K, et al., Anti-inflammatory and anti-nociceptive activities of methanolic extract of the leaves of Fraxinus floribunda Wallich, Afr J Tradit Complement Altern Med, 4 (4), (2007) 411-416.
  • Chamlagai D & Singh B, Study of invitro anti-inflammatory activity of ethnomedicinal plants of Sikkim Viscum articulatum and Acorus calamus, Asian J Pharm Clin Res, 9 (3) (2016) 119-122.
  • Sinha S, Murugesan T, Maiti K, et al., Evaluation of antiinflammatory potential of Bergenia ciliata Sternb. rhizome extract in rats, J Pharm Pharmacol, 53 (2) (2001), 193-196.
  • Turner N J, Counter-irritant and other medicinal uses of plants in Ranunculaceae by native peoples in British Columbia and neighbouring areas, J Ethnopharmacol, 11 (2) (1984)181-201.
  • Hao D, Gu X, Xiao P, et al., Chemical and biological research of Clematis medicinal resources, Chin Sci Bull, 58 (10) (2013) 1120-1129.
  • Chawla R, Kumar S & Sharma A, The genus Clematis (Ranunculaceae): Chemical and pharmacological perspectives, J Ethnopharmacol, 143 (1) (2012) 116-150.
  • Peng C, Perera P K, Li Y M, et al., Anti-inflammatory effects of Clematis chinensis Osbeck extract (AR-6) may be associated with NF-κB, TNF-α, and COX-2 in collageninduced arthritis in rat, Rheumatol Int, 32 (10) (2012) 3119- 3125.
  • Tadele A, Asres K, Melaku D, et al., In vivo antiinflammatory and antinociceptive activities of the leaf extracts of Clematis simensis Fresen, Ethiop Pharm J, 27 (2009) 33-41.
  • Li R W, Myers S P, Leach D N, et al., A cross-cultural study: anti-inflammatory activity of Australian and Chinese plants, J Ethnopharmacol, 85 (1) (2003) 25-32.
  • Yesilada E & Küpeli E, Clematis vitalba L. aerial part exhibits potent anti-inflammatory, antinociceptive and antipyretic effects, J Ethnopharmacol, 110 (3) (2007) 504- 515.
  • Yeşilada E, Üstün O, Sezik E, et al., Inhibitory effects of Turkish folk remedies on inflammatory cytokines: interleukin-1α, interleukin-1β and tumor necrosis factor α, J Ethnopharmacol, 58 (1) (1997) 59-73.
  • http://www.sikkimforest.gov.in (retrived on 05.06.2019).
  • Pan Z H, Li Y, Liu J L, et al., A cytotoxic cardenolide and a saponin from the rhizomes of Tupistra chinensis, Fitoterapia, 83 (8) (2012) 1489-1493.
  • Heinrich M, Ankli A, Frei B, et al., Medicinal plants in Mexico: Healers' consensus and cultural importance, Soc Sci Med, 47 (11) (1998)1859-1871.
  • Phillips O, Gentry A H, Reynel C, et al., Quantitative ethnobotany and Amazonian conservation, Conserv Biol, 8 (1) (1994) 225-248.
  • Friedman J, Yaniv Z, Dafni A, et al., A preliminary classification of the healing potential of medicinal plants, based on a rational analysis of an ethnopharmacological field survey among Bedouins in the Negev Desert, Israel, J Ethnopharmacol, 16 (2-3) (1986) 275-287.
  • Singh B, Qubra K, Chamlagai D, et al., An evaluation of Invitro anti-inflammatory activity of ethnomedicinal plants Dendrocnide sinuata (Blume) chew and Chenopodium ambrosioides (L.), Int J Pharm Sci Rev Res, 54 (2) (2019) 20-24.
  • Shinde U A, Kulkarni K R, Phadke A S, et al., Mast cell stabilizing and lipoxygenase inhibitory activity of Cedrus deodara (Roxb.) Loud. wood oil, Indian J Exp Biol, 37 (1999) 258-261.
  • Mizushima Y & Kobayashi M, Interaction of anti‐inflammatory drugs with serum proteins, especially with some biologically active proteins, J Pharm Pharmacol, 20 (3) (1968)169-173.
  • Sakat S S, Juvekar A R & Gambhire M N, In-vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis Corniculata Linn., Int J Pharm Pham Sci, 2 (1) (2010) 146-55.
  • Parekh J & Chanda S, In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants, Turk J Biol, 31 (1) (2007) 53-58.
  • Abbasi A M, Guo X & Nazir A, Preliminary assessment of phytochemical contents and antioxidant properties of Pistacia integerrima fruit, Pak J Pharm Sci, 28 (4) (2015) 1187-1194.
  • Pradhan B K & Badola H K, Ethnomedicinal plant use by Lepcha tribe of Dzongu valley, bordering Khangchendzonga Biosphere Reserve, in north Sikkim, India, J Ethnobiol Ethnomed, 4 (1) (2008) 22.
  • Mizushima Y, Inhibition of protein denaturation by antirheumatic or antiphlogistic agents, Arch Int Pharmacodyn Ther, 149 (1964) 1-7.
  • Leelaprakash G, & Dass S M, Invitro anti-inflammatory activity of methanol extract of Enicostemma axillare, Int J Drug Dev Res, 3 (3) (2011) 189-196.
  • Chawla R, Kumar D, Godara A, et al., Analgesic and Antiinflammatory Activities of Clematis erecta Aerial Parts, Indian J Pharm Sci, 79 (3) (2017) 474-477.
  • Haloi P, Ahmed W, Roy K, et al., Antidiabetic activity of hydroethanolic flower extract of Tupistra nutans Wall ex. Lindl. in Streptozotocin (STZ) and alloxan induced diabetic rats. ResearchGate, (2015) P75.
  • Khatoon U, Sharma L, Manivannan S, et al., Proximate analysis, elemental profiling and antioxidant activities of Tupistra nutans wall grown in Sikkim Hills, India, J Pharmacogn Phytochem, 7 (2) (2018) 3630-3633.
  • Xiang L, Yi X, Wang Y, et al., Antiproliferative and antiinflammatory polyhydroxylated spirostanol saponins from Tupistra chinensis, Sci Rep, 6 (2016) 31633.
  • Dunder R J, Luiz-Ferreira A, Almeida A C A D, et al., Applications of the hexanic fraction of Agave sisalana Perrine ex Engelm (Asparagaceae): control of inflammation and pain screening, Memórias do Instituto Oswaldo Cruz, 108 (3) (2013) 263-271.
  • Verma S & Nath L K, Analytical Standards for the Flowers of Tupistra nutans Wall. -A Rare Medicinal Plant of Sikkim Himalayan Region. Scholar Research Library, 8 (19) (2016) 48-56.
  • Subba B & Thapa S, Analysis of phytoconstituents and biological activities on the selected medicinal plants of Dolakha and Sindhual Chowk district of Nepal, J Inst Sci Technol, 22 (2) (2018) 140-147.
  • Doughari J H, Phytochemicals: Extraction methods, basic structures and mode of action as potential chemotherapeutic agents, In: Phytochemicals-A global perspective of their role in nutrition and health, edited by V Rao, In Tech Open, (2012). DOI: 10.5772/26052
  • Yan L H, Xu L Z, Wang Z M, et al., Two new phenolic glycosides from the stems of Clematis parviloba. Yao xue xue bao= Acta pharmaceutica Sinica, 45 (12) (2010) 1527- 1532.
  • Zhu F, Du B & Xu B, Anti-inflammatory effects of phytochemicals from fruits, vegetables, and food legumes: A review, Crit Rev Food Sci Nutr, 58 (8) (2018) 1260-1270.
  • Zhang L, Ravipati A S, Koyyalamudi S R, et al., Antioxidant and anti-inflammatory activities of selected medicinal plants containing phenolic and flavonoid compounds, J Agri Food Chem, 59 (23) (2011) 12361-12367.
  • Baumann J, Bruchhausen F V & Wurm G, Flavonoids and related compounds as inhibitors of arachidonic acid peroxidation, Prostaglandins, 20 (4) (1980) 627-639.
  • Fu Q, Zan K, Zhao M, et al., Triterpene saponins from Clematis chinensis and their potential anti-inflammatory activity, J Nat Prod, 73 (7) (2010) 1234-1239.