Journal article Open Access

Orthogonalization of far-field detection in tapered optical fibers for depth-selective fiber photometry in brain tissue

Bianco; Pisanello; Balena; Montinaro; Pisano; Spagnolo; Sabatini; De Vittorio; Pisanello

The field of implantable optical neural interfaces has recently enabled the interrogation of neural circuitry with both cell-type specificity and spatial resolution in sub-cortical structures of the mouse brain. This generated the need to integrate multiple optical channels within the same implantable device, motivating the requirement of multiplexing and demultiplexing techniques. In this, we present an orthogonalization method of the far-field space to introduce mode-division demultiplexing for collecting fluorescence from implantable tapered optical fibers. This is achieved by exploiting the correlation between the transversal wavevector ᵅ8;ᵆ1; of the guided light and the position of the fluorescent sources along the implant, an intrinsic property of the taper waveguide. On these bases, we define a basis of orthogonal vectors in the Fourier space, each of which is associated to a depth along the taper, to simultaneously detect and demultiplex the collected signal when the probe is implanted in fixed mouse brain tissue. Our approach complements existing multiplexing techniques used in silicon-based photonics probes with the advantage of a significant simplification of the probe itself.

M.B., B.S., Fi.P., A.B., and Fe.P. acknowledge funding from the European Research Council under the European Union's Horizon 2020 Research and Innovation Program under Grant Agreement No. 677683. M.D.V., Fe.P. and A.B. acknowledge from the European Union's Horizon 2020 Research and Innovation Program under Grant Agreement No. 828972. Fi.P., M.D.V. and Fe.P. acknowledge that this project has received funding from the European Union's Horizon 2020 Research and Innovation Program under Grant Agreement No 101016787. M.P. and M.D.V. acknowledge funding from the European Research Council under the European Union's Horizon 2020 Research and Innovation Program under Grant Agreement No. 692943. M.P., Fe.P., and M.D.V. were funded by the U.S. National Institutes of Health (Grant No. 1UF1NS108177-01).
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