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Published January 26, 2022 | Version v1

Mechanical characterisation of the developing cell wall layers of tension wood fibres by Atomic Force Microscopy

  • 1. LMGC, Univ. Montpellier, CNRS, Montpellier, France
  • 2. CTM, Univ. Montpellier, Montpellier, France
  • 3. INRAE, ONF, BioForA, Orléans, France

Contributors

  • 1. LMGC, Univ. Montpellier, CNRS, Montpellier, France

Description

This dataset corresponds to the Arnould et al. (2022) paper (available at https://www.biorxiv.org/content/10.1101/2021.09.23.461481v1.full) on the mechanical characterization of developing cell wall layers of tension wood fibers by Atomic Force Microscopy. It contains all raw AFM files (Bruker format, readable by the free software Gwyddion for example) corresponding to mechanical measurements of poplar reaction wood cells (clone 717-1B4) along 3 radial lines, starting from the cambium. Each cell is identified by its "macroscopic" distance from the cambium (value in µm in the name of each file corresponding to the displacement of the sample in the AFM) which was corrected after using the AFM optical images (tif files). Some files, with a -z extension after the distance value, correspond to a zoom into the cell wall. The data also contain measurements made for mechanical calibration on epoxy embedded Kevlar fibers, controlled measurements in the embedding resin between each radial line and measurements in normal wood cells. Two csv files containing final data extracted from AFM measurements that give the value of the indentation modulus and the relative thickness to cell diameter ratio (by AFM and by optical microscope) in each cell wall layer as a function of cambium distance are also provided.

Files

AFM files.zip

Files (323.9 MB)

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Additional details

Funding

Agence Nationale de la Recherche
StressInTrees - Coupled physiological and micro-mechanical approach on maturation stress generation in tension wood ANR-12-BS09-0004