Engineering of Fully Humanized and Vascularized 3D Bone Marrow Niches Sustaining Undifferentiated Human Cord Blood Hematopoietic Stem and Progenitor Cells

Data underlying the figures in the publication “Engineering of fully humanized and vascularized 3D bone marrow niches sustaining undifferentiated human cord blood hematopoietic stem and progenitor cells”, published in J Tissue Eng., 2021, 12, 2041731421044855.

DOI:10.1177/20417314211044855

Table of Contents:

1. P12-03_TP01_Suppernatant: Enzyme-linked immunosorbent assay of VEGF concentration in vascularized and not vascularized BM niches.

2. P12_WholeNiche: qPCR data of vascular and osteogenic markers in vascularized and not vascularized BM niches.

3. FACs-Overview-SN_relativ: FACS data showing haematopoietic cell populations in the supernatant after HSC cocoulture in vascularized and not vascularized BM niches.

4. FACs-Overview-niche_Relativ: FACS data showing haematopoietic cell populations in the niche after HSC cocoulture in vascularized and not vascularized BM niches.

5. CFU: Colony formation statistics after HSC cocoulture from vascularized and not vascularized BM niches.

6. Cell cycle: Cell cycle statistics of HSPCs after HSC cocoulture from vascularized and not vascularized BM niches.

7. P12_03_AfterHSPC-vs-BeforeHSPCs: qPCR data of vascular and osteogenic markers in vascularized and not vascularized BM niches after and before HSC cocoulture.

8. Scheme: Vector graphic of experimental setup.

9. Images engineered niches: Folder containing images of engineered niches.