Effect of mobile phase composition and pH on thin layer chromatographic behaviour of amino acids

Analytical Research Laboratory, Department of Chemistry, Hindu College, B-3, Jigar Colony,

Moradabad-244 001, India

Munuscript received 13 June 2000, revised 19 October 2000, accepted 24 February 2001

The chromatographic behaviour of twentyfour amino acids has been studied on plain silica gel-G layers in one-component, two component (butanol-acetic acid) and three-component (acetone-benzene-acetic acid) mobile phases in varying ratios. The effects of pH and composition of the mobile phase and also of the mole fraction of each component on R_F values are discussed. The mechanism of migration is explained in terms of adsorption on silica gel-G and the polarity of the mobile phase.