Urine proteomics in the diagnosis of stable angina

Background: We have previously described a panel of 238 urinary polypeptides specific for established severe coronary artery disease (CAD). Here we studied this polypeptide panel in patients with a wider range of CAD severity.

Methods: We recruited 60 patients who underwent elective coronary angiography for investigation of stable angina. Patients were selected for either having angiographic evidence of CAD or not (NCA) following coronary angiography (n = 30/30; age, 55 ± 6 vs. 56 ± 7 years, P = 0.539) to cover the extremes of the CAD spectrum. A further 66 patients with severe CAD (age, 64 ± 9 years) prior to surgical coronary revascularization were added for correlation studies. The Gensini score was calculated from coronary angiograms as a measure of CAD severity. Urinary proteomic analyses were performed using capillary electrophoresis coupled online to micro time-of-flight mass spectrometry. The urinary polypeptide pattern was classified using a predefined algorithm and resulting in the CAD₂₃₈ score, which expresses the pattern quantitatively.

Results: In the whole cohort of patients with CAD (Gensini score 60 [40; 98]) we found a close correlation between Gensini scores and CAD₂₃₈ (ρ = 0.465, P < 0.001). After adjustment for age (β = 0.144; P = 0.135) the CAD₂₃₈ score remained a significant predictor of the Gensini score (β =0.418; P < 0.001). In those with less severe CAD (Gensini score 40 [25; 61]), however, we could not detect a difference in CAD₂₃₈ compared to patients with NCA (−0.487 ± 0.341 vs. −0.612 ± 0.269, P = 0.119).

Conclusions: In conclusion the urinary polypeptide CAD₂₃₈ score is associated with CAD burden and has potential as a new cardiovascular biomarker.