Published June 15, 2014 | Version v1
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SCREENING TEST FOR DETECTION OF COLORADO POTATO BEETLE (Leptinotarsa decemlineata Say ) SENSITIVITY TO ABAMECTIN

  • 1. University of Novi Sad, Faculty of Agriculture, Trg D. Obradovića 8, Novi Sad, Serbia
  • 2. Faculty of Agronomy, Cara Dušana 34, Čačak, Serbia

Description

Screening test was carried out to assess the sensitivity to 19 filed populations of Colorado potato beetle (Leptinotarsa decemlineata
Say.) to abamectin during 2008 (Futog, Čenej, Vilovo, Kovilj and Čurug), 2009 (Stepanovićevo, Gložane, Žabalj, Sirig, Šajkaš, Ljutovo, Šimuže, Bačko Gradište, Novo Miloševo i Kikinda) and 2012 (Zmajevo, Despotovo, Vilovo and Čurug). The test provided a quick assessment of sensitivity of overwintered adults to abamectin (commercial insecticide Abastate) applied at label rate (0.75 l/ha), two-fold (1.5 l/ha) and five-fold higher rates (3.75 l/ha), by soaking method (5 sec). Mortality was assessed after 72 h of exposition. Results were corrected for the mortality in the control and presented as efficacy (%) after the treatment. According to IRAC (method 011), with slight corrections, the sensitivity of Colorado potato beetle was classified by scale 1-5 (1 - highly sensitive
population (E= 100%); 2 - sensitive (100 > E ≥ 95%); 3 – slightly resistant (95>E ≥90%); 4 - resistant (90>E ≥50%); 5 – highly resistant (E < 50%). From five tested populations in 2008, four were highly sensitive (E=100%) to abamectin at label rate and one was sensitive (E=97.5%). In 2009, out of ten tested populations, three were slightly resistant (E=92.6-94.6%), five were resistant (E=64.6-88.7%) and two were highly resistant (E=22.5-48.3%) to abamectin at label rate. Out of four tested populations in 2012, one was highly sensitive (E=100%), one was sensitive (E=95.8%) and two were slightly resistant (E=90.6-94.2%). The survival of adults in treatments with higher rates of abamectin, that the label one, prove that there is a part of population which has endured changes in susceptibility or selection to resistance.

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References

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