Oxidation of isoniazid and glutathione with bromamine-T

Department of Studies in Chemistry, University of Mysore, Manasagangothri, Mysore-570 006, India

E-mail : yath@chemist.com

Manuscript received 17 July 2000, revised 21 March 2001, accepted 12 May 2001

Bromamine-T oxidizes isoniazid and glutathione stoichiometrically in pH 5 acetate buffer medium with four- and ten-electron change, respectively. Back-titration procedures have been developed for the determination of isoniazid and glutathione. The methods have been successfully employed for the determination of isoniazid in pharmaceutical preparations and also for the estimation of metal complexes of glutathione. A direct titration for the determination of glutathione with a visual end-point is developed involving a one-electron change corresponding to the oxidation of thiol group to disulfide.