Data for the "Discovery of Dehydroamino Acid Residues in the Capsid and Matrix Structural Proteins of HIV-1"

Bottom-up mass spectrometry-based proteomic analysis (trypsin) was performed on four biological replicates of HIV-1 virions. These virions were isolated from HEK293T cells transfected with a HIV-1 proviral plasmid derived from the pNL4-3 molecular clone, rendered biosafe due to inactivating point mutations in both the env and vpr reading frames. There are 8 total spectra, 4 are from unlabeled aliquots of sample, and 4 are from aliquots of sample treated with glutathione to label dehydroamino acids (Spectra can be accessed on MassIVE (MSV000088220). All data was analyzed using MetaMorpheus version 0.0.319 (https://github.com/smith-chem-wisc/MetaMorpheus). Provided here are the results of this analysis.