Published December 24, 2021 | Version v1

Non-centrosomal microtubules at kinetochores promote rapid chromosome biorientation during mitosis in human cells

  • 1. Wadsworth Center, New York State Department of Health, Albany, NY, USA
  • 2. Department of Mathematics and the NSF-Simons Center for Multiscale Cell Fate Research, University of California, Irvine, CA, USA
  • 3. Laboratory of Chemistry and Cell Biology, The Rockefeller University, New York, NY, USA
  • 4. Courant Institute and Department of Biology, New York University, New York, NY, USA

Description

Kinetochore tracking data associated with  "Non-centrosomal microtubules at kinetochores promote rapid chromosome biorientation during mitosis in human cells" by Renda, Miles, et al., Current Biology, 2022.

Contact Alexey Khodjakov at alexey.khodjakov@health.ny.gov for any questions or processing routines.

Each file contains coordinates of centrioles and kinetochores in a single cell. 

Key to variables:

ao -- time point corresponding to anaphase onset

cell_id -- comprises three parts: cell type_treatment_number (NaN when only first 15 min of prometaphase were tracked).

centrioles -- coordinates of centrioles. Two pages correspond to the two centrosomes, first three columns are X-Y-Z of the mother and the second three columns are X-Y-Z of the daughter centrioles

kinetochores -- coordinates of kinetochores. Each page corresponds to a chromosome. X-Y-Z coordinates of sister kinetochores are in columns 1-3 and 4-6.

neb -- time point corresponding to nuclear envelope breakdown.

total_chrs -- number of chromosomes in the cell

tracked -- number of tracks obtained in the cell

Notes

supported by NIH GM130298

Files

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