Published December 22, 2021 | Version v1

Lock-in and Standard DPC raw images associated to publication 10.1364/PRJ.445896

  • 1. Chiara
  • 2. Damien
  • 3. Timothé
  • 4. Christophe

Description

Raw images obtained with helicam C3 in a DPC microscope setup. The raw images were used for the figures in the publication Chiara Bonati, Damien Loterie, Timothé Laforest, Christophe Moser. Lock-in incoherent differential phase contrast imaging. Photonics Research, 2022, 10(1):237.

Contents of the data set:

  1. Contrast comparison: images used for Figure 4. Several USAF targets of different height, introducing varying phase differences. Samples were imaged under both standard and lock-in DPC.
    1. Lock-in DPC: lock-in DPC image sets. Each image was collected over four cycles of 140 us each.
      1. 5_mrad: 5 mrad sample
        • Background: no sample image
          • I: Frame I
          • Q: Frame Q
        • Sample: sample image
          • I: Frame I
          • Q: Frame Q
      2. 13dot8_mrad: 13.8 mrad sample
      3. 15dot1_mrad: 15.1 mrad sample
      4. 18dot9_mrad: 18.9 mrad sample
      5. 35dot2_mrad: 35.2 mrad sample
      6. 50dot5_mrad: 50.5 mrad sample
    2. Standard DPC: Same set of images, under standard DPC conditions. Each illumination (left and right) was recorded for 1 us.
      1. 5_mrad: 5 mrad sample
        • Background: no sample image
          • L: Image under left illumination
          • R: Image under right illumination
        • Sample: sample image
          • L: Image under left illumination
          • R: Image under right illumination
      2. 13dot8_mrad: 13.8 mrad sample
      3. 15dot1_mrad: 15.1 mrad sample
      4. 18dot9_mrad: 18.9 mrad sample
      5. 35dot2_mrad: 35.2 mrad sample
      6. 50dot5_mrad: 50.5 mrad sample
  2. Reconstruction: Image sets used for figures 5 and 6
    1. USAF_50dot5mrad: Image sets of a 50.5 mrad glass USAF target.
      1. Illumination: Folder containing the back-focal plane characterisation of the imaging system, used to compute the transfer functions for phase reconstruction.
        • BFP: the back-focal plane aperture of the 0.4 NA objective
        • P_1: reduced pupil used in the experiments
        • s_L: left source
        • s_R: right source
      2. Lock-in DPC: lock-in DPC image set. The equivalent exposure time was 464 us.
      3. Standard DPC: standard DPC image set. Each illumination was exposed for 8 us.
    2. Onion: Image sets of onion epidermal cells.
      1. Lock-in DPC: lock-in DPC images for varying equivalent exposure times.
        • 104 us
        • 120 us
        • 136 us
        • 152 us
        • 168 us
        • 184 us
        • 200 us
        • 216 us
        • 232 us
        • 248 us
      2. Standard DPC: Standard DPC image set at 7 us exposure time

 

 

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Cites
Journal article: 10.1364/PRJ.445896 (DOI)