Secretome of human adipose-derived mesenchymal stem cell relieves pain and neuroinflammation independently of the route of administration in experimental osteoarthritis
Authors/Creators
- 1. Dipartimento di Scienze Farmacologiche e Biomolecolari, University of Milano, Milano, Italy
- 2. IRCCS Istituto Ortopedico Galeazzi, Milano, Italy
- 3. Dipartimento di Scienze Biomediche Chirurgiche e Odontoiatriche, University of Milano, Milano, Italy; IRCCS Istituto Ortopedico Galeazzi, Milano, Italy
Description
The aim of this study was to explore the efficacy of the hASC-CM (human Adipose-derived Stem Cells conditioned medium) to control pain and neuroinflammation in an animal model of OA. OA was induced in mice by intra-articular monosodium-iodoacetate (MIA) injection. Once hypersensitivity was established, hASC-CM was injected by IA, IPL and IV route and its effect monitored over time. Neuroinflammation in nerve, dorsal root ganglia and spinal cord was evaluated measuring proinflammatory markers and mediators by RT-qPCR. A single injection with hASC-CM induced a fast and long lasting antihyperalgesic and antiallodynic effect.
The IV route of administration appeared to be the most efficacious although all the treatments were effective. The
effect on pain correlated with the ability of hASC-CM to reduce the neuroinflammatory condition in both the
peripheral and central nervous system.
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