Antimicrobial Activity of Seed Extracts of Prosopis africana on Selected Microorganisms

To investigate the antimicrobial activity of seed of Prosopis afriacana on clinical isolates of Klebsiella pneumoniae, Pseudomonas aeruginosa and Microsporum canis, agar well diffusion method was used. Extraction using various solvents (distilled water, ethanol and methanol) was done by cold maceration method. Qualitative analysis using the various solvent extracts showed the presence of tannins, flavonoids, alkaloids and phenols in all solvent extracts. The quantitative analysis showed that aqueous and ethanol seed extracts were higher in alkaloids (22.20% and 24.49%) respectively, methanol seed extracts was higher in tannin (1300 mg/100g). All the extracts showed antimicrobial activity against the tested organisms with average diameter zone of inhibition ranging between 6.00-18.00 mm. The minimum inhibitory concentration ranged between 100 mg/ml-200 mg/ml, the minimum bactericidal concentration was at 250 mg/ml for the bacterial isolates and the minimum fungicidal concentration was between 200 mg/ml – 250 mg/ml on the fungal isolate. The results obtained in this work showed that Prosopis africana can be useful in treating ailments caused by pathogenic organisms selected in this work.