Illumina TruSeq stranded mRNA sequences of Rhynchosporium commune isolate UK7 in vitro

Transcription profiles were generated from the Rhynchosporium commune isolate UK7. Cultures were grown either on Luria-Bertani (LBA) or Potato Dextrose Broth (PDB) media. Total RNA was extracted using TRIzol (Invitrogen Inc.) following the manufacturer’s recommendations. Experiments were conducted in triplicates. RNA integrity and quantity was assessed on a Bioanalyzer 2100 (Agilent) and a Qubit fluorometer (Life Technologies) and a Bioanalyzer 2100. Libraries were prepared using the TruSeq stranded mRNA sample prep kit (Illumina Inc.). Total RNA was ribosome-depleted by using polyA selection and reverse-transcribed into double-stranded cDNA.