Published November 17, 2021 | Version v1
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ENZYMATIC CHANGES DURING LETHAL AND SUBLETHAL EXPOSURES OF GLYPHOSATE (41% SL) ON FRESHWATER FISH CIRRHINUS MRIGALA

Authors/Creators

  • 1. Department of Zoology & Aquaculture, Acharya Nagarjuna University, Nagarjuna Nagar 522510, Guntur, Andhra Pradesh, India

Description

The lethal and sublethal effect of the Glyphosate (41% SL) on certain enzymes in tissue such as AAT (Aspartate Amino
Transferase), ALAT (Alanine Amino Transferase), LDH (Lactate Dehydrogenase) and ACP (Acid Phosphatase) of the
freshwater fish, Cirrhinus mrigala during and after the cessation of the exposure were observed. Ten fish were exposed
chronically to lethal and sublethal concentrations for a period of 4 days were sacrificed and tissues such as brain, gill, liver,
kidney and muscle were analysed for biochemical parameters and remaining five fish were maintained in clean well water
for a period of seven days without test substance being spilled in the water and its tissues were collected after reversal
period for biochemical analysis. Marked changes were observed in the biochemical parameters of the fish exposed and
after the cessation of the exposure

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References

  • Agbor, G. A., Taga, I., Nguindex, D. R., Zaidi, M. A., Lehman, L., Altosaar, I., & Ngogang, J. Y. (2011). Effect of iodine supplementation on antioxidant status of normal and alloxan monohydrate in toxicated rats. International Journal of Pharmacology, 7(6), 726-731.
  • Aprea, C., Terenzoni, B., De Angelis, V., Sciarra, G., Lunghini, L., Borzacchi, G., Salvan, A. (2004). Evaluation of skin and respiratory doses and urinary excretion of alkylphosphates in workers exposed to dimethoate during treatment of olive trees. Archives of Environmental Contamination and Toxicology, 48(1), 127-134.
  • Barse, A., Chakrabarti, T., Ghosh, T., Pal, A., & Jadhao, S. (2006). One-tenth dose of LC50 of 4-tert-butylphenol causes endocrine disruption and metabolic changes in Cyprinus carpio. Pesticide Biochemistry and Physiology, 86(3), 172-179.
  • Blahova, J., Modra, H., Sevcikova, M., Marsalek, P., Zelnickova, L., Skoric, M., & Svobodova, Z. (2014). Evaluation of biochemical, haematological, and histopathological responses and recovery ability of common carp (Cyprinus carpio L.) after acute exposure to atrazine herbicide. BioMed Research International, 2014.
  • Bodansky, A., Hallman, L., & Bonoff, R. (1932). Phosphatase studies. 1. Determination of inorganic phosphate. Beer's law and interfering substances in the KuttnerLichtenstein method. Journal of Biological Chemistry, 99, 197-206.
  • Borggaard, O. K., & Gimsing, A. L. (2008). Fate of glyphosate in soil and the possibility of leaching to ground and surface waters: a review. Pest Management Science: Formerly Pesticide Science, 64(4), 441-456.
  • Connolly, A., Jones, K., Galea, K. S., Basinas, I., Kenny, L., McGowan, P., & Coggins, M. (2017). Exposure assessment using human biomonitoring for glyphosate and fluroxypyr users in amenity horticulture. International journal of Hygiene and Environmental Health, 220(6), 1064-1073.
  • Connolly, A., Leahy, M., Jones, K., Kenny, L., & Coggins, M. A. (2018). Glyphosate in Irish adults A pilot study in 2017. Environmental Research, 165, 235-236.
  • Conrad, A., Schröter-Kermani, C., Hoppe, H.-W., Rüther, M., Pieper, S., & Kolossa-Gehring, M. (2017). Glyphosate in German adults–Time trend (2001 to 2015) of human exposure to a widely used herbicide. International Journal of Hygiene and Environmental Health, 220(1), 8-16.
  • Curwin, B. D., Hein, M. J., Sanderson, W. T., Striley, C., Heederik, D., Kromhout, H., Alavanja, M. C. (2007). Urinary pesticide concentrations among children, mothers and fathers living in farm and non-farm households in Iowa. The Annals of Occupational Hygiene, 51(1), 53-65.
  • Das, P. C., Ayyappan, S., Jena, J., & Das, B. (2004). Acute toxicity of ammonia and its sub‐lethal effects on selected haematological and enzymatic parameters of mrigal, Cirrhinus mrigala (Hamilton). Aquaculture Research, 35(2), 134-143.
  • Desai, B. N. (2006). Effects of Agro-Chemicals on the physiological stress on teleost fish. Ph.D. Thesis. The Maharaja Sayajirao University of Baroda, Vadodara, India
  • Economist, T. (2016). Fog of uncertainty-regulators are arguing over the safety of glyphosate, the world's top weedkiller. March, 3, 2016.
  • Flack, S., Goktepe, I., Ball, L. M., & Nylander-French, L. A. (2008). Development and application of quantitative methods for monitoring dermal and inhalation exposure to propiconazole. Journal of Environmental Monitoring, 10(3), 336-344.
  • Gabriel, U., Akinrotimi, O., & Ariweriokuma, V. (2012). Changes in metabolic enzymes activities in selected organs and tissue of Clarias gariepinus exposed to cypermethrin. Journal of Chemical Engineering, 1(1), 25-30.
  • Hakim, D. (2017). Monsanto weed killer Roundup faces new doubts on safety in unsealed documents. New York Time.
  • Israel, M. A., Chidobem, J. I., & Onyemaechi, E. C. Oxidative stress, haematology and histology biomarkers of African clariid catfish clarias gariepinus (Burchell 1822) to glyphosate-based herbicide GBHG,'Glycot'.
  • Kamalaveni, K., Gopal, V., Sampson, U., & Aruna, D. (2003). Recycling and utilization of metabolic wastes for energy production is an index of biochemical adaptation of fish under environmental pollution stress. Environmental Monitoring and Assessment, 86(3), 255-264.
  • Khan, S., & Sharma, N. (2012). A study on enzymes acid phosphatase and alkaline phosphatase in the liver & kidney of fish Gambusia affinis exposed to the chlorpyrifos, an organophosphate. International Journal of Pharmaceutical Sciences Review and Research, 13(1), 88-90.
  • Krüger, M., Schledorn, P., Schrödl, W., Hoppe, H.-W., Lutz, W., & Shehata, A. A. (2014). Detection of glyphosate residues in animals and humans. Journal of Environmental Anals Toxicolology, 4(2), 1-5.
  • Landrigan, P. J. (2018). Pesticides and human reproduction. JAMA Internal Medicine, 178(1), 26-27.
  • Magar, R., & Shaikh, A. (2012). Biochemical changes in proteins and amino acids in Channa punctatus in responses to sub-lethal treatment with the insecticide malathion. Trends in Life Sciences, 1(3), 19-23.
  • Mesnage, R., Defarge, N., De Vendômois, J. S., & Seralini, G. (2015). Potential toxic effects of glyphosate and its commercial formulations below regulatory limits. Food and Chemical Toxicology, 84, 133-153.
  • Mesnage, R., Moesch, C., Grand, R., Lauthier, G., Vendômois, J., Gress, S., & Séralini, G. (2012). Glyphosate exposure in a farmer's family. Journal of Environment Protection, 3(9), 1001
  • Naveed, A., Janaiah, C., & Venkateshwarlu, P. (2011). The effects of lihocin toxicity on protein metabolism of the fresh water edible fish, Channa punctatus (Bloch). Journal of Toxicology and Environmental Health Sciences, 3(1), 018-023.
  • Orrego, R., Guchardi, J., Beyger, L., Krause, R., & Holdway, D. (2011). Comparative embryotoxicity of pulp mill extracts in rainbow trout (Oncorhynchus mykiss), American flagfish (Jordanella floridae) and Japanese medaka (Oryzias latipes). Aquatic Toxicology, 104(3-4), 299-307.
  • Patil, V., Kadam, G., & Raut, P. (2014). Enzymological alterations produced by chronic malathion exposure in freshwater crab, paratelphusa (barytelphusa) jacquemontii. International Journal of Science, Environment and Technology, 3(1), 303-313.
  • Rao, J. V. (2006). Sublethal effects of an organophosphorus insecticide (RPR-II) on biochemical parameters of tilapia, Oreochromis mossambicus. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 143(4), 492-498.
  • Reddy, M. S., Murthy, B. N., Venkateswarlu, Y., & Rao, K. (1985). Toxicity of insecticide phosphamidon on tissue carbohydrate catabolism of penaeid prawn Penaeus indicus (H. Milne Edwards). Journal of Scientific and Industrial Research, 1, 206-207
  • Rodwell, V., Martin, D., Mayg, P., & Garnner, D. (1983). Harper's review of Biochemistry. DW Martin, 292- 295.
  • Sharma, N. (2014). Assessment of the changes in some diagnostic enzymes in fish Gambusia affinis treated with pesticide chlorpyrifos. International Journal of Research in Pharmcology and Pharmacotherapeutics, 3(4), 205-210.
  • Sreenivasan, R., Moorthy, P. K., & Deecaraman, M. (2010). Variations in the enzyme activity of gills and hemolymph in cypermethrin, a pesticide treated freshwater female field crab, Spiralothelphusa hydrodroma (Herbst). International Journal of Sustainable Agriculture, 2(1), 10-15.
  • Tilak, K., Raju, P. W., & Butchiram, M. (2009). Effects of alachlor on biochemical parameters of the freshwater fish, Channa punctatus (Bloch). Journal of Environmental Biology, 30(3), 420-426.
  • Vasantharaja, C., Pugazhendy, K., & Meenambal, M. (2014). Studies on impact of cypermethrin on acid and alkaline phosphatase activity in the selected organs of freshwater fish Cirrhinus mrigala (Hamilton) and the protective effect of Cardiospermum halicacabum. International Research Journal of Pharmaceutical, 5(9), 667-670.
  • Velmurugan, B., Selvanayagam, M., Cengiz, E. I., & Uysal, E. (2008). Levels of transaminases, alkaline phosphatase, and protein in tissues of Clarias gariepienus fingerlings exposed to sublethal concentrations of cadmium chloride. Environmental Toxicology: An International Journal, 23(6), 672-678.
  • Venkatesan. S, K. P., C. Vasantharaja, M, Meenambal, D. Sangeetha, and Prabakaran, S. (2012). Impact of atrazine on acid and alkaline phosphatase activities and protective role of Spirulina in various organs of fish Cyprinus carpio. Linn. International Journal Environmental Biology, 2(1), 31-35.
  • Vitali, M., Protano, C., Del Monte, A., Ensabella, F., & Guidotti, M. (2009). Operative modalities and exposure to pesticides during open field treatments among a group of agricultural subcontractors. Archives of Environmental Contamination and Toxicology, 57(1), 193-202.
  • Yildirim, M. Z., Benlı, A. Ç. K., Selvı, M., Özkul, A., Erkoç, F., & Koçak, O. (2006). Acute toxicity, behavioral changes, and histopathological effects of deltamethrin on tissues (gills, liver, brain, spleen, kidney, muscle, skin) of Nile tilapia (Oreochromis niloticus L.) fingerlings. Environmental Toxicology: An International Journal, 21(6), 614-620.

Subjects

Zoology
10.5281/zenodo.5707245