Synthesis and characterization of N-substituted benzimidazole derivatives and study of their antibacterial and antifungal activity

Organic Chemistry Division, School of Advanced Sciences, VIT University, Vellore-632 014,

Tamilnadu, India

E-mail : dominic.y@vit.ac.in

Medical Biotechnology Division, School of Bioscience and Technology, VIT University,

Vellore-632 014, Tamilnadu, India

N-(4-(1H-Benzo[d]imidazol-1-yl)phenyl)-3-nitrobenzamide, N-(4-(1H-benzo[d]imidazol-1-yl)phenyl)-4- chlorobenzamide, 4-(1H-benzo[d]imidazol-1-yl)benzenamine and N-(4-(1H-benzo[d]imidazol-1-yl)phenyl)-4-bromobenzamide were synthesized and characterized by IR and NMR techniques. These N-substituted benzimidazole derivatives were screened for their antibacterial activity by Well diffusion method against Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus) (Gram-positive) and Escherichia coli (E. coli), Klebsiella (Gram-negative) bacterial strains. The compounds tested were found to have good antibacterial activity against all bacteria. The antifungal activity of the compounds was tested against Aspergillus flavus (A. flavus) and Aspergillus niger (A. niger) fungal strains. Antifungal results indicated that 4-(1H-benzo[d]imidazol-1-yl)benzenamine and N-(4-(1H-benzo[d]imidazol-1-yl)phenyl)-4-bromobenzamide derivatives showed better activity than the reference drug.