Time Dependent Analysis of Rat Microglial Markers in Traumatic Brain Injury Reveals Dynamics of Distinct Cell Subpopulations
Creators
- 1. University of Texas Health Science Center at Houston
Description
The repository contains seven zip files for flow cytometry data for rat microglia following Controlled cortical impact (CCI) collected at seven time points: 3 hours, 1 day, 2 days, 7 days 14 days, 21 days and 28 days.
Each file contains 24 CSV files converted from the raw fcs file and text files that contain meta data about the file.
Each file name depicts the information of whether it is Panel A (M1 in the file name) or Panel B (M2 in the file name) measurement and whether the measurement was conducted on the ipsilateral (ipsi) or contralateral (contra) side of the CCI, or sham conducted on the ipsilateral (ipsi) or contralateral (contra) sides.
Below is the description of the columns of each file:
|
File column name |
Original name |
Panel A (M1) context |
PAnel B (M2) context |
Description |
|
F0 |
FSC-A |
FSC-A |
FSC-A |
Forward scattering Area |
|
F1 |
FSC-H |
FSC-H |
FSC-H |
Forward scattering Height |
|
F2 |
FSC-W |
FSC-W |
FSC-W |
Forward scattering Width |
|
F3 |
SSC-A |
SSC-A |
SSC-A |
Side scattering area |
|
F4 |
FITC-A |
FITC-A |
FITC-A |
Fluorescein isothiocyanate |
|
F5 |
PE-A |
CD32 |
CD200R |
Protein expression |
|
F6 |
PerCP-Cy5-5-A |
- |
CD163 |
Protein expression |
|
F7 |
PE-Cy7-A |
CD11 |
CD11 |
Protein expression |
|
F8 |
BV421-A |
P2Y12 |
P2Y12 |
Protein expression |
|
F9 |
BV510-A |
- |
- |
Dye |
|
F10 |
Alexa Fluor 647-A |
CD86 |
RT1B |
Protein expression |
|
F11 |
APC-Cy7-A |
CD45 |
CD45 |
Protein expression |
|
F12 |
Event Time |
Event Time |
Event Time |
Event Time |
Files
14_days.zip
Files
(244.6 MB)
| Name | Size | Download all |
|---|---|---|
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md5:81f8ffa11f70186adfea07277f76a76d
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33.1 MB | Preview Download |
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md5:94878c8c38f0bb8bc9ca8266b6d25f61
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md5:7c843500b1c5dc563604d41a964ddce8
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md5:4d8866649dd149485a22e3e68889ae25
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md5:86238f1d02bcffbc6560c2c4502049e7
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md5:c6ddd28251f6f53e673c02f5fdbb4d38
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md5:fac93e735772a8744b9a133a6d4b6796
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15.0 kB | Download |