Published August 24, 2021 | Version v1
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Impact of Nordic Walking training program on inflammasome activation in elderly women

Authors/Creators

  • 1. Laboratory of Experimental Biochemistry and Molecular Biology, IRCCS Istituto Ortopedico Galeazzi, Milano, Italia
  • 2. Department of Physical Education and Lifelong Sports, Poznań University of Physical Education, Poznań, Polska; Doctoral School, Gdańsk University of Physical Education and Sport, Gdańsk, Polska
  • 3. Department of Physiology, Gdańsk University of Physical Education and Sport, Gdańsk, Polska
  • 4. Laboratory of Experimental Biochemistry and Molecular Biology, IRCCS Istituto Ortopedico Galeazzi, Milano, Italia; Vita-Salute San Raffaele University, Milano, Italia
  • 5. Department of Athletics, Strength and Conditioning, Poznań University of Physical Education, Poznań, Polska
  • 6. Laboratory of Experimental Biochemistry and Molecular Biology, IRCCS Istituto Ortopedico Galeazzi, Milano, Italia; Department of Athletics, Strength and Conditioning, Poznań University of Physical Education, Poznań, Polska

Description

Patients and methods: 64 elderly women were randomly assigned to either the experimental (EXP, n= 29) and control group (CTRL, n= 35). Subjects from EXP completed 12 weeks of moderate aerobic training program, whereas CTRL did not performe any activity. Blood samples were taken at baseline, before and after the first and the last Nordic walking unit. Inflammasome activation status was assessed through the analysis of whole blood NLRP3 and TLR4 expression by RT-qPCR and the application of a normalization study to define the most reliable normalization strategy. Plasma levels of inflammasome-related cytokines IL-1β, IL-6, IL-18, and TNFα were assayed by a multiplex immunoassay.

Results:The increment of IL-1β and TNFα concentrations was observed in response to the first and last unit of NW. NLRP3 and TLR4 were decreased before the last training session.

Notes

This study was supported by the National Science Center (Poland), project no. NCN 2018/29/B/NZ7/02094.

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