neerjavashist/Gut_Microbiome_Konzo: GutMicrobiomeInKonzo

The detailed code used for the analysis of the gut microbiome of children from the DRC. DNA from stool samples was extracted using the Qiagen QIAMP PowerFecal Kit and was sequenced using shotgun metagenomic sequenceing. The subsequent fastq files (available on Sequence Read Archive (SRA) with BioProject ID PRJNA752006) were analyzed using Kraken 2 and Bracken pipeline, and the functional capacity was analyzed using the KEGG database. The code is used to determine differences in the bacterial profile of the gut microbiome of individuals affected and unaffected with konzo from difference regions of the DRC.