Improved library preparation protocols for amplicon sequencing-based noninvasive fetal genotyping for RHD-positive D antigen-negative alleles
- 1. National Center For Child Health and Development
- 2. Kitasato University Graduate School of Medical Sciences*
- 3. Showa University School of Medicine*
- 4. Jikei University School of Medicine
Description
We aimed to simplify our fetal RHD genotyping protocol by changing the method to attach Illumina's sequencing adaptors to PCR products from the ligation-based method to a PCR-based method, and to improve its quantitative accuracy by introducing unique molecular indexes, which allow us to count the numbers of DNA fragments used as PCR templates and to minimize the effects of PCR and sequencing errors. Both of the newly established protocols reduced time and cost compared with our conventional protocol. Removal of PCR duplicates using UMIs reduced the frequencies of erroneously mapped sequences reads likely generated by PCR and sequencing errors. The modified protocols will help us facilitate implementing fetal RHD genotyping for East Asian populations into clinical practice.
Files
fetal_RHD_genotyping_script_ver210805.txt
Additional details
Related works
- Is cited by
- 10.1186/s13104-021-05793-4 (DOI)
- Is source of
- 10.5061/dryad.4tmpg4fb3 (DOI)