Technical note Open Access

A clone resource of cereal heavy-metal associated (HMA) domains— putative targets of plant pathogen effector proteins

Langner, Thorsten; Kellner, Ronny; Harant, Adeline; Terauchi, Ryohei; Kamoun, Sophien

In plants, heavy metal associated (HMA) domains have recurrently integrated into NLR immune receptors and are thought to be commonly targeted by pathogen effectors. Here, we describe a clone resource of 151 HMA domains of the cereal grasses rice (Oryza sativa), foxtail millet (Setaria italica) and wheat (Triticum aestivum) in the yeast two-hybrid “bait” plasmid pGBKT7. We aim to provide a community resource of HMA domains for rapid protein-protein interactions screens and other functional analyses.

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Langner_et_al_2021_HMA_Library.docx
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Suplementary_File1_Oryza_sativa_sHMA_full.faa
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Suplementary_File2_Setaria_italica_sHMA_full.faa
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Suplementary_File3_Triticum_aestivum_sHMA_full.faa
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Suplementary_File4_Oryza_sativa_sHMA_cloned.faa
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Suplementary_File5_Setaria_italica_sHMA_cloned.faa
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Suplementary_File7_Oryza_sativa_sHMA_insert_nucleotide.fna
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Suplementary_File8_Setaria_italica_sHMA_insert_nucleotide.fna
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Suplementary_File9_Triticum_aestivum_sHMA_insert_nucleotide.fna
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