Published December 12, 2020 | Version v1
Journal article Open

Métodos de detección del SARS-CoV-2 en pacientes enfermos de COVID-19

Authors/Creators

  • 1. Centro de Investigación en Ciencias Microbiológicas, Posgrado en Microbiología. Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, México
  • 2. Escuela de Ingeniería en Mecatrónica de la Universidad Politécnica de Tlaxcala, Tlaxcala, México

Description

RESUMEN

El brote emergente de la enfermedad por coronavirus 2019 (COVID-19) continúa extendiéndose por todo el mundo. Demostrando su efecto dañino en muchos sistemas y órganos humanos; esto es de gran preocupación para la sociedad en general afectando la vida diaria y la economía mundial. Además, de causar una necesidad sin precedentes de utilizar métodos de diagnóstico rápidos y sensibles para detectar el virus, especialmente cuando las vacunas no están disponibles. Motivo por el cual el objetivo de la presente revisión fue comparar estudios publicados para obtener información sobre los métodos de detección del SARS-CoV-2 como: la reacción en cadena de la polimerasa con transcriptasa inversa (RT-qPCR), la amplificación isotérmica mediada por asa con transcriptasa inversa (RT-LAMP), las pruebas serológicas y los diferentes biosensores como: (i) los biosensores colorimétricos ópticos (fluorescentes), (ii) los biosensores electroquímicos (los potenciométricos y los amperométricos), (iii) los biosensores basados en aptámeros y (iv) los polímeros de impresión molecular (PIM). Además, se resumieron las ventajas y desventajas de las plataformas que están en la etapa de desarrollo y el crecimiento como nuevas tecnologías de detección para el diagnóstico del SARS-CoV-2.

 

ABSTRACT

The emerging outbreak of coronavirus disease 2019 (COVID-19) continues to spread around the world. Demonstrating its damaging effect on many systems and human organs; this is of great concern to society likewise it affects their daily life and the world economy. Caused an unprecedented need for rapid diagnostics for rapid and sensitive detection of the virus, especially when the vaccines are not available. This study aimed to compare published studies to obtain information on the detection methods of SARS-CoV-2 such as: the RT-qPCR, the RT-LAMP, the serological tests, and the different biosensors such as: (i) the optical colorimetric biosensors (fluorescent), (ii) the electrochemical biosensors (potentiometric and amperometric), (iii) the biosensors based on aptamers, and (iv) the molecular impress polymer (MIP). Also was summarized the advantages and disadvantages of new platforms that find in the development and growth stage as new detection technologies for diagnosing the SARS-CoV-2.

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Identifiers

ISSN
2594-0627
URL
https://www.aytbuap.mx/aytbuap-520/m%C3%A9todos-de-detecci%C3%B3n-del-sars-cov-2-en-pacientes-enfermos-de-covid-19
URL
https://eoi.citefactor.org/10.11235/BUAP.05.20.02

Related works

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Journal article: https://repositorioinstitucional.buap.mx/handle/20.500.12371/9780 (URL)

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