High-Speed Atomic Force Microscopy Highlights New Molecular Mechanism of Daptomycin Action

Data underlying the figures in the publication “High-speed atomic force microscopy highlights new molecular mechanism of daptomycin action”, published in Nat Commun, 2020, 11, 6312. https://doi.org/10.1038/s41467-020-19710-z

Table of contents:

1. Movie 1; HS-AFM movie of the first minutes after exposure to sub-MIC Dap on a POPG supported membrane. Guides to the eye highlights those oligomers identifiable. Movie parameters: frame rate 33 ms; full image of 90 nm x 65 nm and 256x180 pixels; colour depth 8bit (256 values); full colour scale 4 nm.

2. Movie 2; HS-AFM movie after tens of minutes after exposure to sub-MIC Dap that shows diffusing dimples on a POPG supported membrane which interact by swinging trajectories. Movie parameters: frame rate 83 ms; full image of 150nm x 150nm and 256x256 pixels; colour depth 8bit (256 values); full colour scale 4 nm.

3. Movie 3; HS-AFM movie of the first minutes after exposure to over-MIC of a POPG supported membrane. A flow of material is visualized thanks to the motion of the ripples, it starts at the lm3m cubic phase (left) and ends at a tubulation (right). Movie parameters: frame rate 456 ms; full image of 400nm x 400nm and 300x300 pixels; colour depth 8bit (256 values); full colour scale 16 nm.

4. Movie 4; HS-AFM movie of the cyclic accumulation of material in the pores created on TOCL/POPG supported membranes under the exposure of the outer leaflet to supplementary quantities of Dap added to the imaging solution. The process seems to eject material out of the membrane; see the material that appears next to the pore at 1.30s. Movie parameters: frame rate 260 ms; zoom of a full image of 140nm x 100nm and 256x180 pixels; colour 29 depth 8bit (256 values); full colour scale 3 nm.