Published June 29, 2021 | Version v1

Figure 5 of 'Structural basis for the specificity of PPM1H phosphatase for Rab GTPases'

Authors/Creators

Description

Raw data for Figure 5 from manuscript 'Structural basis for the specificity of PPM1H phosphatase for Rab GTPases', Dieter Waschbüsch, Kerryn Berndsen, Pawel Lis, Axel Knebel, Yuko P. Y. Lam, Dario R. Alessi, Amir R. Khan

Figure 5BE Malachite.xlsx:

Numerical data for the charts shown in Fig.5B and Fig.5E.

 

Figure 5C.tif:
Coomassie stained gel of recombinant PPM1H WT and mutant proteins shown in Fig.5C

 

Figure 5D 700.png:
Top to bottom:
1) Total LRRK2 blot shown in Fig.5D
2) HA blot for PPM phosphatases (PPM constructs) shown in Fig.5D
3) GAPDH loading control for blots in Fig.5D
4) HA blot for Rab10 shown in Fig.5D

 

Figure 5D 800.png:

1) Phospho-Rab10 pThr73 blot shown in Fig.5D

 

Figure 5E.tif:
Coomassie stained gel of recombinant PPM1H WT and mutant proteins shown in Fig.5E

 

Figure 5F 700 Rab8A.png:
Top to bottom (Rab8A)

1) Total LRRK2 blot shown in Fig.5F
2) HA blot for PPM phosphatases (PPM constructs) shown in Fig.5F
3) GAPDH loading control for blots in Fig.5F
4) HA blot for Rab8A shown in Fig.5F

 

Figure 5F 800 Rab8A.png:

1) Phospho-Rab8A pThr72 blot shown in Fig.5F

 

Figure 5F 700 Rab10.png:

Top to bottom (Rab10)
1) Total LRRK2 blot shown in Fig.5F
2) HA blot for PPM phosphatases (PPM constructs) shown in Fig.5F
3) GAPDH loading control for blots in Fig.5F
4) HA blot for Rab10 shown in Fig.5F

 

Figure 5F 800 Rab10.png:

1) Phospho-Rab10 pThr73 blot shown in Fig.5F

Files

Figure 5C.tif

Files (4.0 MB)

Name Size Download all
md5:1fafb88ae954198765ca6ccdb5e69b24
16.0 kB Download
md5:6cae2f24f330363b52558663dd13bb8d
1.3 MB Preview Download
md5:746fb918c023ed50823c8031524bf516
159.1 kB Preview Download
md5:489607f1c2cc32d3ca76df20f63eeda2
102.6 kB Preview Download
md5:f8e942dacd578402ea1a5bc7d598a55b
1.9 MB Preview Download
md5:73e54f02e1ad3d3de78bf0a5616c8bed
177.2 kB Preview Download
md5:e2f12440b8a11b953fcd473d218c28b9
151.3 kB Preview Download
md5:2a7474179ca8e19f5f5646182640648a
131.2 kB Preview Download
md5:a165b39a523fab2d43098f621cf6c394
94.7 kB Preview Download