Published June 28, 2021 | Version v1

MicroED datasets of hemozoin

  • 1. Diamond Light Source
  • 2. Weizmann Institute of Science
  • 3. STFC
  • 4. University of Vienna

Description

Nineteen diffraction datasets (tilt_04.tar.xz contains a repeat tilt series) were collected using a Talos Arctica operated at 200 kV with a stage temperature of ~80 K. The gun lens was reduced to the minimum (8), the smallest spot size (11) with a C2 aperture of 50 μm was used, and the beam condensed to be 1.78 μm in diameter. The diffraction images were recorded by a Ceta-D camera in MRC format, using the EPU-D data collection software. The diffraction patterns were collected at a nominal camera length of 1.05 m. The dose rate under these conditions was 0.04 e- Å-2 s-1.

A data processing script is provided to index, integrate and scale the data using DIALS 3.5.

An associated manuscript is in preparation.

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