Synchronized reagent delivery in double emulsions for triggering chemical reactions and gene expression

Data underlying the figures in the publication “Synchronized reagent delivery in double emulsions for triggering chemical reactions and gene expression”, published in Small Methods.

Table of contents:

1. Figure 2C; Origin file containing all data and analysis for Figure 2C. Requires Origin Software to open.

2. Figure 3; FlowJo Workspace for data analysis and fcs raw data acquired from flow cytometer for Figure 3B and 3C. Requires FlowJo software to open.

Raw data file names:

200721 FDG in PVA after prod_FDG  sample 6_006.fcs

200721 FDG in PVA&SDS 4 h after prod_FDG  sample 4 PVA shake_002.fcs

200721 FDG in PVA&SDS 4 h after prod_FDG  sample 4 SDS 001 shake_004.fcs

200721 FDG in PVA&SDS 22 h after prod_FDG  sample 4 PVA shake_002.fcs

200721 FDG in PVA&SDS 22 h after prod_FDG  sample 4 SDS 001 shake_004.fcs

3. Figure 4BC; Origin file containing all data and analysis for Figure 4B and 4C. Requires Origin Software to open.

4. Figure 5; Origin file containing all data and analysis for Figure 5D. Requires Origin Software to open.

File Figure 5_201215-new LUVs 500 mM DH5alpha: FlowJo Workspace for data analysis and fcs raw data acquired from flow cytometer for Figure 5B. Requires FlowJo software to open.

Raw data file names:

201215-new LUVs 500 mM-DH5alpha_sample 1 18h after prod_012.fcs

201215-new LUVs 500 mM-DH5alpha_sample 3 18h after prod- 05%SDS_018.fcs

5. Figure S2; PDF files, report from DLS instrument.

6. Figure S4; Excel file containing the values extracted from microscopy images for Figure S4.

7. Figure S5; FlowJo Workspace for data analysis and fcs raw data acquired from flow cytometer for Figure S5. Requires FlowJo software to open.

Raw data file names:

200721 FDG in PVA after prod_FDG  sample 6_006.fcs

200721 FDG in PVA&SDS 4 h after prod_FDG  sample 4 SDS 001 shake_004.fcs

200721 FDG in PVA&SDS 4 h after prod_FDG  sample 5 SDS 01 shake_008.fcs

200721 FDG in PVA&SDS 4 h after prod_FDG  sample 6 SDS 0001 shake_006.fcs

200721 FDG in PVA&SDS 22 h after prod_FDG  sample 4 SDS 001 shake_004.fcs

200721 FDG in PVA&SDS 22 h after prod_FDG  sample 5 SDS 01 shake_008.fcs

200721 FDG in PVA&SDS 22 h after prod_FDG  sample 6 SDS 0001 shake_006.fcs

8. Figure S6; Origin file containing all data and analysis for Figure S6 left-Requires Origin Software to open-, and Excel file containing the values from 96 well plate measurements used for Figure S6 right.

9. Figure S7; Excel files containing the values from 96 well plate measurements used for Figure S7 left, and the values extracted from microscopy images for Figure S7 right.

10. Figure S9; FlowJo Workspace for data analysis and fcs raw data acquired from flow cytometer for Figure S9. Requires FlowJo software to open.

Raw data file names:

201215-new LUVs 500 mM-DH5alpha_sample 1 3h30min after prod_005.fcs

201215-new LUVs 500 mM-DH5alpha_sample 1 18h after prod_012.fcs

201215-new LUVs 500 mM-DH5alpha_sample 1 30min after prod_001.fcs

201215-new LUVs 500 mM-DH5alpha_sample 3 3h30min after prod - 05%SDS_008.fcs

201215-new LUVs 500 mM-DH5alpha_sample 3 18h after prod- 05%SDS_018.fcs