Published June 18, 2021 | Version v1
Dataset Open

Scripts and raw data for comparing 2D vs 3D image analysis of zebrafish embryo microscopic data

Authors/Creators

  • 1. Karlsruhe Institute of Technology

Description

Raw data and MatLab scripts used for image analysis of RNA polymerase II with serine 5 phosphorylation in the C-terminal domain (CTD) of the subunit 1 (Pol II Ser5P) in a fixed zebrafish embryo, comparing a 2D vs 3D approach to segment out the Pol II Ser5P clusters. Pol II Ser5P was labeled by immunofluorescence, microscopy images were acquired by instant-SIM microscopy and analyzed using MatLab scripts and the bioformats importer.

Notes

Steps needed to carry out the analysis: 1. (if necessary) Install bfmatlab from OME, add it to the MatLab path 2. Run comparison_script.m

Files

ImageAnalysis_2Dvs3D.zip

Files (3.0 GB)

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md5:170e86e34f8bd74bc644ec0babed2388
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