G-quadruplex in the gene of the large subunit of plant RNA polymerase II: billion years old story

Supplementary material to the journal article

Consist of:

Supplementary material S1: Analyzed RPB1 sequences in 40 plant species together with detailed characteristics and G-quadruplex prediction using four different computational approaches.

Supplementary material S2: G4 locus is the most conserved within the RPB1 gene (40 bp long potential G4 locus is the most conserved site in the whole ~ 6000 bp long RPB1 gene. See the histogram below the alignment: the position of the G4 locus is depicted, together with the horizontal red dashed line indicating relative nucleotide conservation among aligned sequences of RPB1)

Supplementary material S3: Multiple alignment of G4 locus of RPB1 paralogs in Arabidopsis thaliana centered to G4 locus of RPB1 

Supplementary material S4: Modelled 3D structure of G4 from Bathycoccus prasinos in PDB format

Supplementary material S5: Gel electrophoresis and ThT staining of the selected G4-forming sequences

Supplementary material S6: All analyzed RPB1 sequences in FASTA format

Supplementary material S7: Aligned RPB1 sequences in FASTA format

Supplementary material S8: RPB1 paralogs in Arabidopsis thaliana

Supplementary material S9: Spectral composition of light used in the UV experiment. Analysis of emitted light was performed by Ocean Optics (HR4000CG-UV-NIR, USA) device.

Supplementary material S10: Difference CD spectra - comparison without and with previous UV treatment