Published June 17, 2021 | Version v1
Dataset Open

Effect of hexanediol treatment on clusters of RNA polymerase II in zebrafish embryo primary cell culture

Authors/Creators

  • 1. Karlsruhe Institute of Technology

Description

This data set assesses changes in RNA polymerase II clusters upon treatment with 3% 1,6-hexanediol in zebrafish embryos. RNA polymerase was labeled by immunofluorescence, microscopy images were acquired by instant-SIM microscopy and analyzed using MatLab scripts and the bioformats importer. This data set contains the raw image data as well as all further analysis scripts.

Notes

Steps needed to carry out the analysis: 1. Install bfmatlab from OME, add it to the MatLab path 2. Extract the image data by running the script iSIM_extraction_tiff.m 3. Analyze the extracted data by running the script Hexanediol_Cluster_Analysis.m 4. Generate example images by running the script ExampleImages.m

Files

HE_Analysis.zip

Files (786.3 MB)

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