Published June 17, 2021
| Version v1
Dataset
Open
Analysis of RNA polymerase II phosphorylation in STimulated Emission Double Depletion (STEDD) microscopy images
Description
This data set includes the raw image data and MatLab analysis scripts to assess the relative distribution of RNA polymerase II C-terminal domain serine 5 and serine 2 phosphorylation in zebrafish embryos. Phosphorylated polymerase II was labeled by immunofluorescence, microscopy images were acquired by STEDD microscopy and analyzed using MatLab scripts. Images were recorded in the laboratory of Uli Nienhaus at Karlsruhe Institute of Technology.
Notes
Steps needed to carry out the analysis:
1. Extract the image data by running the script STEDDistributionsExtraction.m
2. Analyze the extracted data by running the script STEDD_AggregateAnalysis.m
3. Generate example images by running the scriptExampleAggregateZooms.m