Journal article Open Access

Pixelwise H-score: a novel digital image analysis based-metric to quantify membrane biomarker expression from immunohistochemistry images

Ram, Sripad; Vizcarra, Pamela; Whalen, Pamela; Deng, Shibing; Painter, CL; Jackson-Fisher, Amy; Pirie-Shepherd, Steven; Xia, Xiaoling; Powell, Eric

The dataset consists of whole slide image scans of immunohistochemistry images of human tumor resections. Specifically, there are 3 different sets of images pertaining to biomarkers PD-L1 (n = 24), 5T4 (n = 21) and P-cadherin (n = 30). The IHC images for PD-L1 and P-cadherin are in svs file format, while the IHC images for 5T4 are in NDPI file format. All the images can be opened using the following software packages: 

1. Aperio ImageScope software; free image viewer,  https://www.leicabiosystems.com/digital-pathology/manage/aperio-imagescope/ 

2. QuPath software; free viewer and image analysis software, https://qupath.github.io/

3. HALO software; requires a license, http://www.indicalab.com

4. Visiopharm software; requires a license, http://www.visiopharm.com


 

Files (38.0 GB)
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01and02 lungT IHC 5T4.ndpi
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03and04 lungT IHC 5T4.ndpi
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15and16 lungT IHC 5T4.ndpi
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17and18 lungT IHC 5T4.ndpi
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19and20 lungT IHC 5T4.ndpi
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21and22 lungT IHC 5T4.ndpi
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23and24 lungT IHC 5T4.ndpi
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25 and Control lungTN IHC 5T4.ndpi
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F00082146.svs
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F00082175.svs
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F00082454.svs
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F00082455.svs
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F00082463.svs
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PFZ083 MOS634-PD ML1610205 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610206 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610207 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610208 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610209 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610210 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610211 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610212 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610213 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610214 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610215 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610216 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610217 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610218 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610219 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610220 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610221 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610222 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610223 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610224 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610225 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610226 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610227 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610228 20161010 PD-L1.svs
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PFZ083 MOS634-PD ML1610229 20161010 PD-L1.svs
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README.txt
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SampleList.pdf
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