Published May 17, 2021
| Version v1
Dataset
Open
Reconstructing single-cell karyotype alterations in colorectal cancer reveals punctuated and gradual diversification patterns
Authors/Creators
- Yannik Bollen1
- Ellen Stelloo1
- Petra van Leenen1
- Myrna van den Bos1
- Bas Ponsioen1
- Bingxin Lu2
- Markus van Roosmalen1
- Ana Bolhaqueiro3
- Christopher Kimberley4
- Max Mossner4
- William Cross4
- Nicolle. Besselink1
- Bastiaan van der Roest1
- Sander Boymans1
- Koen C. Oost1
- Sippe de Vries1
- Holger Rehmann1
- Edwin Cuppen1
- Susanne Lens1
- Geert Kops3
- Wigard Kloosterman1
- Leon Terstappen5
- Chris Barnes2
- Andrea Sottoriva6
- Trevor Graham7
- Hugo Snippert1
- 1. Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University, The Netherlands
- 2. University College London, UK
- 3. Hubrecht Institute-KNAW, The Netherlands
- 4. Barts Cancer Institute, UK
- 5. University of Twente, Enschede, The Netherlands
- 6. The Institute of Cancer Research, London, UK
- 7. Barts Cancer Institute, London, UK
Description
We developed ‘3D Live-Seq’, a protocol that enables the reconstruction of evolving tumor cell karyotypes across multiple consecutive cell generations by integrating live-cell imaging data of tumor organoid outgrowth and whole-genome sequencing data of each imaged cell. Using patient-derived colorectal cancer organoids and freshly isolated tumor tissue, we demonstrate that chromosomal copy-number alterations of varying complexity are prevalent and can arise within a few cell generations.Here deposited are the discrete copy-number values per bin for each single cell or bulk sequenced sample.
Files
dataset_575B_20201205.csv
Files
(8.4 MB)
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