Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model
Authors/Creators
- 1. University of Belgrade – Faculty of Chemistry, Department of Biochemistry, Belgrade, Serbia
- 2. Resaerch Group Innovative Testing in Life Sciences & Chemistry, Utrecht University of Applied Sciences, Utrecht, the Netherlands
- 3. Institute for Oncology and Radiology of Serbia, Belgrade, Serbia
- 4. Resaerch Group Innovative Testing in Life Sciences & Chemistry, Utrecht University of Applied Sciences, Utrecht, the Netherlands; Institute for Risk Assessment Sciences, Utrecht University, Utrecht, the Netherlands
- 5. Institute for Risk Assessment Sciences, Utrecht University, Utrecht, the Netherlands
Description
In this study, two intestinal models were employed to assess the modulatory potential of a major kiwifruit
allergen on the innate immunity of epithelial cells. Effects of Act d 1 were analyzed in terms of gene expression
and structural changes of tight junction (TJ) proteins, as well as up-regulation of pro-inflammatory cytokines in
Caco-2 cells and, for the first time, in mouse-derived intestinal 2-dimensional (2D) organoids.
Biologically active Act d 1 induced up-regulation of TJ genes for CLDN-2, CLDN-3, CLDN-4, ZO-1, and on the
protein level induced release of pro-inflammatory cytokines IL-1β, TNFα and IL-33 in both employed model
systems. In 2D-organoids, active Act d 1 impaired the TJ protein networks of E-cadherin, claudin-3, and ZO-1.
2D-organoids generated from mouse intestine are a promising new model system for the assessment of allergen-
induced intestinal cell responses and a useful tool for mitigation of risks associated with novel food
proteins.
Notes
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Additional details
Related works
- Is source of
- 1756-4646 (ISSN)
Funding
- Ministry of Education, Science and Technological Development
- Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance 172049