Detection of Neoplasms by Metagenomic Sequencing of Cerebrospinal Fluid

Detection of Neoplasms by Metagenomic Sequencing of Cerebrospinal Fluid

Wei Gu, MD, PhD^1,2*, Andreas M. Rauschecker, MD, PhD³, Elaine Hsu, BS¹, Kelsey C. Zorn, BS⁴, Yasemin Sucu, BS¹, Scot Federman, BS¹, Allan Gopez, BS¹, Shaun Arevalo, BS¹, Hannah A. Sample, BS⁴, Eric Talevich, PhD⁵, Eric D. Nguyen, MD, PhD ¹, Marc Gottschall, BS¹, Bardia Nourbakhsh, MD, MAS⁶, Carl A. Gold, MD, MS⁷, Bruce A.C. Cree, MD, PhD, MAS⁸, Vanja Douglas, MD⁸, Megan B. Richie, MD⁸, Maulik P. Shah, MD, MHS⁸, S. Andrew Josephson, MD⁸, Jeffrey M. Gelfand, MD, MAS⁸, Steve Miller, MD, PhD ¹, Linlin Wang, MD¹, Tarik Tihan, MD, PhD⁹, Joseph L. DeRisi, PhD^4,10, Charles Y. Chiu, MD, PhD^11,12, Michael R. Wilson, MD, MAS⁸*

¹Department of Laboratory Medicine, University of California San Francisco, CA 94107, USA

²Department of Pathology, Stanford University, CA 94305, USA

³Department of Radiology and Biomedical Imaging, University of California San Francisco, CA 94107, USA

⁴Department of Biochemistry and Biophysics, University of California San Francisco, CA 94107, USA

⁵DNANexus, Mountain View, CA 94040, USA

⁶Department of Neurology, Johns Hopkins University, MD 21287, USA

⁷Department of Neurology, Stanford University, CA 94305, USA

⁸Weill Institute for Neurosciences, Department of Neurology, University of California San Francisco, CA 94107, USA

⁹Department of Pathology, University of California San Francisco, CA 94107, USA

¹⁰Chan Zuckerberg Biohub, San Francisco, CA 94107, USA

¹¹UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA 91407, USA

¹²Department of Medicine, Division of Infectious Diseases, University of California San Francisco, CA 94107, USA

* Corresponding authors:

Michael Wilson, MD, MAS

UCSF Department of Neurology

675 Nelson Rising Lane, NS212

San Francisco, CA 94158

Michael.Wilson@ucsf.edu

Wei Gu, MD, PhD

Stanford University, Department of Pathology

3373 Hillview Ave, 218

Palo Alto, CA 94304

650-723-1914

weigu@stanford.edu