Journal article Open Access

ALS and FTD-associated missense mutations in TBK1 differentially disrupt mitophagy

Harding, Olivia; Evans, Chantell S.; Ye, Junqiang; Cheung, Jonah; Maniatis, Tom; Holzbaur, Erika L.F.

TANK-binding kinase 1 (TBK1) is a multi-functional kinase with an essential role in mitophagy, the selective clearance of damaged mitochondria. More than 90 distinct mutations in TBK1 are linked to amyotrophic lateral sclerosis (ALS) and fronto-temporal dementia (FTD), including missense mutations that disrupt the ability of TBK1 to dimerize, associate with the mitophagy receptor optineurin (OPTN), auto-activate, or catalyze phosphorylation. We investigated how ALS-associated mutations in TBK1 affect Parkin-dependent mitophagy using imaging to dissect the molecular mechanisms involved in clearing damaged mitochondria. Some mutations cause severe dysregulation of the pathway, while others induce limited disruption. Mutations that abolish either TBK1 dimerization or kinase activity were insufficient to fully inhibit mitophagy, while mutations that reduced both dimerization and kinase activity were more disruptive. Ultimately, both TBK1 recruitment and OPTN phosphorylation at S177 are necessary for engulfment of damaged mitochondra by autophagosomal membranes. Surprisingly, we find that ULK1 activity contributes to the phosphorylation of OPTN in the presense of either WT- or kinase inactive TBK1. In primary neurons, TBK1 mutants induce mitochondrial stress under basal conditions; network stress is exacerbated with further mitochondrial insult. Our study further refines the model for TBK1 function in mitophagy, demonstrating that some ALS-linked mutations likely contribute to disease pathogenesis by inducing mitochondrial stress or inhibiting mitophagic flux. Other TBK1 mutations exhibited much less impact on mitophagy in our assays, suggesting that cell-type specific effects, cumulative damage, or alternative TBK1-dependent pathways such as innate immunity and inflammation also factor into the development of ALS in affected individuals.

We would like to thank Dr. Mariko Tokito for re-engineering the TBK1 constructs with various tags, and the entire Holzbaur group for invaluable discussion. We gratefully acknowledge Project ALS for initiating this collaboration and Project ALS (Grant ID 2018-03) and NINDS (NS060698) for supporting this work. The study is also funded by the joint efforts of The Michael J. Fox Foundation for Parkinson's Research (MJFF) and the Aligning Science Across Parkinson's (ASAP) initiative. MJFF administers the grant ASAP-000350 on behalf of ASAP and itself. C.S.E. was supported by the Howard Hughes Medical Institute Hanna H. Gray Fellowship.
Files (40.8 GB)
Name Size
1C_FR-halo-TBK1_dsRed2-mito_YFP-Parkin_cccp 2cell1.ome.tiff
md5:e8f87550c7042a010e3a735b23edf904
112.4 MB Download
1E_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 WT 3 cell2.ome.tiff
md5:ae1d5482ddc83d95f184619231544530
153.7 MB Download
1F_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 e696k 1 cell1.ome.tiff
md5:e0fcaa45012e2b7825e85c5e8eadf9d9
166.2 MB Download
2A_MR_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 m559r 5cell1.ome.tiff
md5:f6242b48a12afca5b14ead2852f4ed64
114.6 MB Download
2A_RQ_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 r357q 3cell1.ome.tiff
md5:2a1908b820b0cdc2b480926ddaef0301
227.9 MB Download
2A_WT_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_sBFP2-mito_WT 3 cell1.ome.tiff
md5:5238957426e885f315927b98f78c3011
117.1 MB Download
3A_DN_d135n_plateA_3cell1.ome.tiff
md5:d48f0129eb1ba705eeca917e3d94337a
164.8 MB Download
3A_GR_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_sBFP2-mito_g217r 4cell1.ome.tiff
md5:d9c47a6904e06a8f3075e29ed7409827
123.7 MB Download
3A_IT_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 i257t 2CELL1.ome.tiff
md5:08b166f562da4be94c977397cfca174c
122.8 MB Download
3A_R2H_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_sBFP2-mito r228h 2cell1.ome.tiff
md5:518780a12f7126cb14a795f4a20848c0
147.3 MB Download
3A_RH_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 r47h 4cell1.ome.tiff
md5:d6d40d36dc621fb49ac5019dd1dced81
128.0 MB Download
3A_RQ_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 r308q1CELL2.ome.tiff
md5:60312b304da3bb94d56f52ef1e48054b
135.4 MB Download
3A_SC_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 s151c 6cell2.ome.tiff
md5:a9bc08cc2aae04c99d32c58826c416eb
188.2 MB Download
3A_SF_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 s151f 3cell1.ome.tiff
md5:8feee1155ecef5aabe27c97ec15e0898
105.7 MB Download
3A_YC_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_sBFP2-mito y105c 1cell3.ome.tiff
md5:7f0382f219d5777cbc8f2b0b3f1794d8
199.7 MB Download
4A_GR_190811_liveHela_FR-Halo-TBK1_mCh-Parkin_EGFP-OPTN_sBFP2-mito - g217r.tif
md5:3211f029e80605c3bd0b67ad3b803bee
744.1 MB Download
4A_MR_190821_liveHela_FR-Halo-TBK1_mCh-Parkin_EGFP-OPTN_sBFP2-mito - m559r.tif
md5:685f01bce2e54dfd3d3cb1be5033050b
480.1 MB Download
4A_RQ_190828_liveHela_FR-Halo-TBK1_mCh-Parkin_EGFP-OPTN_sBFP2-mito - r357q.tif
md5:d4b7df0e363e5da3bebb7da8bf18df95
648.1 MB Download
4A_WT_190811_liveHela_FR-Halo-TBK1_mCh-Parkin_EGFP-OPTN_sBFP2-mito.mvd2 - WT.tif
md5:326467594a4a2beef1f4f62eb41dec83
752.1 MB Download
4C_200924_hek_lysateVSmitoEnrich_GAPDH.tif
md5:1e7161a786cf37e06432b6ea27e2c0ef
1.7 MB Download
4C_200924_hek_lysateVSmitoEnrich_Parkin1.tif
md5:d8e6ca4707699da9e8ea80f69085e74b
590.2 kB Download
4C_200924_hek_lysateVSmitoEnrich_TBK1.tif
md5:212cb187077cf66ab1133895e0f64cac
763.9 kB Download
4C_200924_hek_lysateVSmitoEnrich_TOM20.tif
md5:4be9c56befd3e5efe5f2483112b7b7ad
1.4 MB Download
5A_191223_WT 3cell1.ome.tif
md5:ffe7b33b1d7d3136393728d586b515d5
86.9 MB Download
5B_191223_g217r 6cell2.ome.tif
md5:670142a0b917aa378e38f3a0acda1925
412.4 kB Download
5C_191223_r357q 3cell1.ome.tif
md5:7e26ab51ed38acec50baf38f138ae6c2
100.6 MB Download
5D_200115_m559rB 2cell2.ome.tiff
md5:dee3f77499bac1033ff62de5be3780fe
69.8 MB Download
5E_210216_d135nB_2cell1.ome.tiff
md5:9b577bebcaf713f5e4e2b010559c5a57
77.2 MB Download
6A_210302_siTBK1_noRescue_ULK101_cccp 2.tif
md5:15827d52fb9c356ee9601284dac49b98
184.0 MB Download
6A_210302_siTBK1_WT_cccp 2.tif
md5:21937a6ee69110d289963d4d52f5f15b
264.0 MB Download
6A_210315_+DN_A 5.tif
md5:da00b2f406b1a6b25905bd243a06b423
408.1 MB Download
6A_210315_+DN_ULK101A 1.tif
md5:2da00e237a9394c7d1b6eb19483463ae
256.0 MB Download
6A_210315_+WT_ULK101A 4.tif
md5:e7c46a3238d2128f361b245ff8d5db43
408.1 MB Download
6A_210315_siTBK1_B 3.tif
md5:71ee2d09043e8e45d7993ec407fbb5cb
344.0 MB Download
6C_190927_helaFixed_EGFP-LC3_dsRed2-mito_FRHalo-TBK1_notag-Parkin_m559r cccp 2.tif
md5:4d6ed894625b948773f6204f55688d05
330.0 MB Download
6C_190927_helaFixed_EGFP-LC3_dsRed2-mito_FRHalo-TBK1_notag-Parkin_WT cccp 2.tif
md5:6d3329fd59e2a6db11c6ab979c1ba149
252.0 MB Download
7A_190927_helaFixed_EGFP-LC3_dsRed2-mito_FRHalo-TBK1_notag-Parkin_cccp - WT cccp.tif
md5:9510406adfad5c4b99f749b7d1918fc3
288.0 MB Download
7A_190927_helaFixed_EGFP-LC3_dsRed2-mito_FRHalo-TBK1_notag-Parkin_cccp.mvd2 - g217r cccp 2.tif
md5:b40fb397c179a2ca8ea097801179a124
276.0 MB Download
7A_191111_m559r 1108_ 2cell1.ome.tiff
md5:fb221656522560b90a9d60e29de7069e
47.9 MB Download
7A_191111_r357q 1108_ 4cell1.ome.tiff
md5:f2ed04d0cd5c37982f05271da6678f71
114.2 MB Download
7A_200113_d135n 2cell1.ome.tiff
md5:32dc8386172afc7022fbad46f2229950
78.7 MB Download
7C_190927_helaFixed_EGFP-LC3_dsRed2-mito_FRHalo-TBK1_notag-Parkin_cccp.mvd2 - WT cccp 2.tif
md5:21698d6b2b07cde13edd80afb7e1b1b9
252.0 MB Download
7D_190927_helaFixed_EGFP-LC3_dsRed2-mito_FRHalo-TBK1_notag-Parkin_cccp.mvd2 - m559r cccp 2.tif
md5:aab9db98e3c45bf956f8192a414e3021
330.0 MB Download
8A_200908_MitoSNAPTMREHaloTBK1M559R_AA 3.ome.tif
md5:1fd86f1431b1a683a632f83c8991bc36
150.7 MB Download
8A_200908_MitoSNAPTMREHaloTBK1M559R_Ctrl 2.ome.tif
md5:c22e098339180a3a51d33923ceeb7529
150.7 MB Download
8A_200908_MitoSNAPTMREHaloTBK1R357Q_AA 6.ome.tif
md5:0a5e4e186bc92bc7e2a92c0885ec26ed
162.8 MB Download
8A_200908_MitoSNAPTMREHaloTBK1R357Q_Ctrl 4.ome.tif
md5:79c324418ba76e63cf14c33d09994e87
144.7 MB Download
8A_200908_MitoSNAPTMREHaloTBK1WT_AA 2.ome.tif
md5:c8d4d61b168ddf3268708c3961f426e0
164.8 MB Download
8A_200908_MitoSNAPTMREHaloTBK1WT_Ctrl 9.ome.tif
md5:128d8127ad948b311ae79417da0868b2
162.8 MB Download
8F_200922_MitoDsRedYFPParkinHaloTBK1WT_AA.ome.tif
md5:82cef2d658aa31099565a6ee414b4c2c
420.2 MB Download
8F_200929_MitoDsRedYFPParkinHaloTBK1M559R_AA 6.ome.tif
md5:974d618139d00e3110fe05e979ff87da
354.2 MB Download
8F_200929_MitoDsRedYFPParkinHaloTBK1M559R_Ctrl 9.ome.tif
md5:ad37bec5fc9cbe2360bb07914104ba22
522.3 MB Download
8F_200929_MitoDsRedYFPParkinHaloTBK1R357Q_AA.ome.tif
md5:4f8be482ad6c400fc034113635a66e86
468.3 MB Download
8F_200929_MitoDsRedYFPParkinHaloTBK1R357Q_Ctrl 11.ome.tif
md5:427d8bfeed153cdff0138e7db3b95f61
456.3 MB Download
8F_200929_MitoDsRedYFPParkinHaloTBK1WT_Ctrl 3.ome.tif
md5:040b3ee31a84b351332d5ffc1941e90a
432.2 MB Download
HardingEtAl_ZenodoMASTER.xlsx
md5:9789e38255118676d89b5d2a7eaed9a9
404.0 kB Download
S1A_191025_SNAP700_TBK1800_SNAP_rgb.tif
md5:0f87cf7d21092a1807be4fa464faca92
2.6 MB Download
S1A_191025_TBK1.tif
md5:69ba6af6b929d16e00286a43b558332a
3.0 MB Download
S1C_190225_EK01.tif
md5:023c1b6b93383b69a36e478007b5455d
5.5 MB Download
S1C_190225_GR02.tif
md5:b98fda3bbad7dd810e4c9a5bc83ee39d
5.5 MB Download
S1C_190225_IT01.tif
md5:c1841fb846ca1810f767be9ca7943b29
5.5 MB Download
S1C_190225_MR02.tif
md5:6b8510d42dc960aa18dc9c0472ee8716
5.5 MB Download
S1C_190225_R2H02.tif
md5:4fc306a9cdeaaec179085897710c1495
5.5 MB Download
S1C_190225_R35Q01.tif
md5:b7bbb08697f94cab17dfed0733f6940d
5.5 MB Download
S1C_190225_RH02.tif
md5:f8ad5b42dff02c5651dbbf9873c4ce56
5.5 MB Download
S1C_190225_RQ02.tif
md5:55d437ad2afa0bc01468b4897b6dc4af
5.5 MB Download
S1C_190225_SC02.tif
md5:b734c032822b44cf877821ac7d6bd59f
5.5 MB Download
S1C_190225_SF02.tif
md5:707d7681eb2d824ebc89ceefcf8c7647
5.5 MB Download
S1C_190225_WT02.tif
md5:90dd7c6b2b0117c56eba112c7d675cd7
5.5 MB Download
S1C_190225_YC02.tif
md5:d5501efea29775828424154bbcbd4fdd
5.5 MB Download
S1D_200703_blot2_Halo.tif
md5:c7a0a3ba5f69adbcf7799905f4fedcde
1.5 MB Download
S1E_200824_d135n_ctrl 4.ome.tiff
md5:21d12f86cc3b46ed8d3997959fdee827
360.4 MB Download
S1E_200824_g217r_ctrl 3.ome.tiff
md5:751c1d37f11d1d5e4804cd35b3bf5479
520.6 MB Download
S1E_200824_m559r_ctrl 3.ome.tiff
md5:6f1b8b0e73e973622b4fbb6f9d006444
288.3 MB Download
S1E_200824_r357q_ctrl 2.ome.tiff
md5:a67c272dbe28bc4674bee69094caf5a8
344.4 MB Download
S1E_200824_WT_ctrl 3.ome.tiff
md5:b8af025daf63b616b61203971c7d58d7
432.5 MB Download
S2A_011320_helaFixed_ringsHiQuality- FR-Halo-OPTN_dsRed2-mito_EGFP-LC3_notag-Parkin_cccp 2cell1.tif
md5:a4471ae73d5f69be78ef0bc7a5ff6b19
142.9 MB Download
S2B_0401_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_Hoes_siTBK1 e696k 5_max.tif
md5:49c8d75a84a22560841fb8febd9943b0
8.0 MB Download
S2B_0506_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 WT 3.tif
md5:c061d4886217f4baf438176f6297f3e7
976.1 MB Download
S2C_190506_fixedHela_TBK1vars FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 m559r 5.tif
md5:3a397c6d4c066ed8e9f9f3143e3de75d
776.1 MB Download
S2C_190506_fixedHela_TBK1vars FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 r357q 2.tif
md5:fe03c163136a00c3ddbf922fcb424351
552.1 MB Download
S2C_190506_fixedHela_TBK1vars FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 WT 4.tif
md5:9e38e30d4c0d4deb811c4445450b03cc
480.1 MB Download
S3A_190506 FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 i257t 6.tif
md5:5368fba0926e7f6288e9873eeb3b61c9
480.1 MB Download
S3A_190506_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 r308q 4.tif
md5:c96ca35b867aa29c3e8cb7be6b445a04
472.1 MB Download
S3A_190506_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 r47h 6.tif
md5:398751658a41c86aa1a31f75f4c44e44
416.1 MB Download
S3A_190506_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 s151c.tif
md5:a069dd6ed45180f1cad815824c55aa1e
392.1 MB Download
S3A_190506_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 s151f.tif
md5:0eecc3061f2a33129bff686dd933efd3
632.1 MB Download
S3A_190705_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_sBFP2-mito g217r 4.tif
md5:820cda8c0a4190508332e20b3802b0e4
520.1 MB Download
S3A_190705_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_sBFP2-mito r228h.tif
md5:ac80a23f8d742a57b7c4eb5a19d2880b
480.1 MB Download
S3A_190705_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_sBFP2-mito y105c 3.tif
md5:52ce8add2249cceaa210b69c8869b927
752.1 MB Download
S3A_191223_sBFP2-mito_YFP-Parkin_TMR-Halo-OPTN_FR-SNAP-TBK1 d135n_plate3 4.tif
md5:50acc1b0087d1377a7f80c74cbbb668b
440.0 MB Download
S3B_181119_08 siTBK1 wtTBK1 cccp deconv.tif
md5:e6ee2cae9edbc20d6bf5ab401ee8ec5f
242.3 MB Download
S3B_200306_FR-SNAP-TBK1_a594-pOPTN_EGFP-OPTN_sBFP2-mito_cccp 2_cell2.ome.tiff
md5:2917186de135d5070ca0244a93672bcc
74.5 MB Download
S4A_200929_d135n 2.ome.tiff
md5:7caf77650cfb39b07c0e4bb688ef9746
60.1 MB Download
S4A_200929_g217r 2.ome.tiff
md5:511eeff90610b01e3328f0d888491910
60.0 MB Download
S4A_200929_m559r 4.ome.tiff
md5:5d319812e5b343f3262ab8f80d92d23c
60.1 MB Download
S4A_200929_r357q 1.ome.tiff
md5:249ab699759fb6d6091a6271bd73be0d
60.0 MB Download
S4A_200929_WT 4.ome.tiff
md5:d77e6adf666926f983afe76e590d53ea
60.1 MB Download
S4C_210308_+MR_cccp.ome.tiff
md5:2bbee9a08112c80129789f2f3f4041e5
416.5 MB Download
S4C_210308_+WT_cccp.ome.tiff
md5:eeccad796b9cf21b78fc475a956cc6c8
328.4 MB Download
S4C_210308_WT cells.ome.tiff
md5:2f089d623ae3f6ca509f0afd1325bba2
208.2 MB Download
S4C_210308_WT cells_cccp.ome.tiff
md5:0cf207cf2c74aad4f53a7ce06043da87
352.4 MB Download
S4C_210315 HEK_RQ_CCCP 2.ome.tiff
md5:71e62a2e0ac26a127dc65d9d304a9224
624.7 MB Download
S5A_190705_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_sBFP2-mito WT 4cell2.ome.tiff
md5:5a3a02ca877af4c755b5fb68f73b8d68
134.7 MB Download
S5A_200306_siTBK1_tmr-Halo-OPTN_YFP-Parkin_sBFP-mito_cccp.tif
md5:2b47e6f5123e408114acbbcff03edab8
448.0 MB Download
S5B_190422_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 i257t.tif
md5:b6d2230f57135bcb33c3c69da40a62f0
560.1 MB Download
S5B_190422_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 r357q 2.tif
md5:4287e6b755689ef4e1a640451b89a5f6
424.1 MB Download
S5B_190422_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 r47h 3.tif
md5:47a0415e12b3d5fec51d2ee8f070e12d
664.1 MB Download
S5B_190422_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 WT 4.tif
md5:78729122a8c686674a26f1d571772f5a
568.1 MB Download
S5B_190506_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 i257t 5.tif
md5:1b95f88fcb812fdf12d3dd8e43c25435
728.1 MB Download
S5B_190506_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 m559r 6.tif
md5:8c712d74700bf836cec8f08cc29d6861
560.1 MB Download
S5B_190506_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 r308q 3.tif
md5:dec6af5fb07aa651eab7a0b378265e38
424.1 MB Download
S5B_190506_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 r47h 5.tif
md5:b5223ce527a7110d2b1e1f3f427cde07
512.1 MB Download
S5B_190506_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 s151c 4.tif
md5:0e686b2c916a1556a2f41eaab0110049
528.1 MB Download
S5B_190506_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 s151f 2.tif
md5:17ecc8c5562d93254f205aedf27bb7ee
608.1 MB Download
S5B_190506_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 s151f 4.tif
md5:f932c29845b9e525f75ccae9d2b8fac2
384.1 MB Download
S5B_190506_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 WT 6.tif
md5:c6616c696467b783b804d070873d66a1
1.0 GB Download
S5B_190513_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 m559r 2.tif
md5:cecfdbef439880094f3d8c2ae7edb85f
376.0 MB Download
S5B_190513_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 r308q 2.tif
md5:418bfe420b44fa869ff59c67c51204b4
496.1 MB Download
S5B_190513_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 r357q 6.tif
md5:7198c97f0e49f69e2924dd6a3d980e19
432.1 MB Download
S5B_190513_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_405-aTOM20_siTBK1 s151c.tif
md5:0716d0de662b407d683eec9d4beb0c5a
376.1 MB Download
S5B_190705_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_sBFP2-mito g217r 7.tif
md5:ea9c8780c49ccf72e18098fc00b148b4
432.1 MB Download
S5B_190705_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_sBFP2-mito y105c 4.tif
md5:1f591d6d714ce1d1f87236f3359570b3
336.0 MB Download
S5B_190715_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_sBFP2-mito g217r 10.tif
md5:015f9d770ae57c09bec06e308d8ccc7b
448.1 MB Download
S5B_190715_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_sBFP2-mito r228h 2.tif
md5:b046cb8faf8ba2151e111bd786e410d7
624.1 MB Download
S5B_190715_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_sBFP2-mito r228h 4.tif
md5:66c1259fcbff3398613a1e33e4a6c3c8
608.1 MB Download
S5B_190715_FR-SNAP_TMR-Halo-OPTN_YFP-Parkin_sBFP2-mito y105c 7.tif
md5:e27d2c3a3eaf7bfb41ad4344f74ae56b
456.1 MB Download
S5B_191223_helaFixed_sBFP2-mito_YFP-Parkin_TMR-Halo-OPTN_FR-SNAP-TBK1_d135n_plate3 3.tif
md5:d2ffb9c58ad1b150516a1adb4e60a9f0
440.0 MB Download
S5B_d135n 1111a 1cell1.ome.tiff
md5:362065d893adffadc61cc7916d4c9f38
90.5 MB Download
S6A_200115_helaFixed_sBFP2-mito_EGFP-OPTN_594-a-pOPTN_FR-Halo-TBK1_notag-Parkin_cccp_g217rA 5.tif
md5:af02990490d1ec79d99035f4b9468ff3
328.0 MB Download
S6A_200115_helaFixed_sBFP2-mito_EGFP-OPTN_594-a-pOPTN_FR-Halo-TBK1_notag-Parkin_cccp_m559rB 4.tif
md5:3a1a8b111e82a66b6161a491a18d45ad
264.0 MB Download
S6A_200115_helaFixed_sBFP2-mito_EGFP-OPTN_594-a-pOPTN_FR-Halo-TBK1_notag-Parkin_cccp_r357qA 3.tif
md5:f4b2fc5c348f168674f707f8c6cd95ce
496.1 MB Download
S6A_200115_helaFixed_sBFP2-mito_EGFP-OPTN_594-a-pOPTN_FR-Halo-TBK1_notag-Parkin_cccp_wt 4.tif
md5:b60bc522649c668fea51cef6a402f6a4
424.1 MB Download
S6A_210216_helaFixed_FR-HaloTBK1_594-apOPTN_egfp-optn_sBFP2-mito_Parkin_cccp_d135nA 4.tif
md5:d4cb48ec41c009047252ae7d8ead55bf
488.1 MB Download
S7A_011320_helaFixed_EGFP-LC3_dsRed2-mito_FRHalo-TBK1_notag-Parkin_cccp_d135n 8.tif
md5:48bdb4a6b39f0a9d5f87b9ca0a87306a
222.0 MB Download
S7A_190927_helaFixed_EGFP-LC3_dsRed2-mito_FRHalo-TBK1_notag-Parkin_cccp_g217r cccp 3.tif
md5:4a33c8f8930b7c08b6a3c7e20f1631c1
366.0 MB Download
S7A_190927_helaFixed_EGFP-LC3_dsRed2-mito_FRHalo-TBK1_notag-Parkin_cccp_r357q cccp 3.tif
md5:f9a57b16b0d0f60e2fbfc0d6e2973803
294.0 MB Download
S7A_190927_helaFixed_EGFP-LC3_dsRed2-mito_FRHalo-TBK1_notag-Parkin_cccp_WT cccp 4.tif
md5:fdc9c5459f5bc054bb322e0cfd92b0e8
306.0 MB Download
S7A_191111_helaFixed_EGFP-OPTN_dsRed2-mito_FR-halo-TBK1_notagParkin_cccp_m559r 1105 4.tif
md5:38bcbfe9d434f548998409f2bea0f1bf
270.0 MB Download
S8A_201005_HippoLysate_TBK1KD_Full Image.tif
md5:045e6805ff3082b1ae096c1fd11c67c8
5.8 MB Download
S8C_201027_HippoLysate_TBK1KD_Full Image.tif
md5:092552549f5b8c3623d4d44075bee09d
11.0 MB Download
175
71
views
downloads
All versions This version
Views 175175
Downloads 7171
Data volume 12.3 GB12.3 GB
Unique views 150150
Unique downloads 2828

Share

Cite as