Published March 22, 2021
| Version v1
Journal article
Open
Colon epithelial cell TGFβ signaling modulates the expression of tight junction proteins and barrier function in mice
Authors/Creators
- 1. Department of Surgery, Section of Surgical Sciences, Vanderbilt University Medical Center, Nashville, TN
- 2. Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN
- 3. Center for Quantitative Sciences, Vanderbilt University Medical Center, Nashville, TN
Description
Supplemental Table 1. Five mice lacking intestinal SMAD4 expression ("KO") and five littermate control mice ("WT") had their colon microbiome composition compared. 16S targeted sequencing was performed on mouse stool pellets ("Pellet") and colon mucosal scrapings ("Scraping") and compared at the phylum, class, order, family, genus, and species levels. Values represent proportion of organisms detected per sample. Genotypes were split evenly between sexes. The five female mice were co-housed and the five male mice were co-housed, with bedding mixed between cages weekly.
Notes
This research was funded in part by National Cancer Institute grants (F32 CA236309; T32 CA106183; R01 CA235016), the Burroughs Wellcome Fund Physician-Scientist Institutional Award #1018894/Vanderbilt Supporting Careers in Research for Interventional Physicians and Surgeons (SCRIPS), the Vanderbilt Ingram Cancer Center (VICC) grant (P30 CA068485), the Vanderbilt Digestive Diseases Research Center Grant (P30 DK058404), and the Department of Veterans Affairs Career Development Award (IK2BX004648).
Files
Files
(128.6 kB)
| Name | Size | |
|---|---|---|
|
md5:51551b678af8270836d42d4c08f9879d
|
128.6 kB | Download |