Published January 19, 2021 | Version v2
Dataset Open

Local Z Projector example dataset and parameters 01

  • 1. Institut Pasteur

Description

Drosophila pupa notum, captured with a laser-scanning confocal microscope (LSM 880, Zeiss). Pixel size 0.188 µm, z step 1 µm.

This dataset  also contains the parameters to generate the reference surface and local projection, also included. It is meant to be used with the Fiji plugin called 'LocalZProjector': https://gitlab.pasteur.fr/iah-public/localzprojector. The two *.localzprojector files can be loaded directly in the plugin to load adequate parameters.

See the accompanying paper: DProj: A toolbox for local 2D projection and accurate morphometrics of large 3D microscopy images.

https://www.biorxiv.org/content/10.1101/2021.01.15.426809v2

This is a downsampled version of the dataset used to generate the figures 1, 2 and 3 in this paper, as well as the comparison in table 1.

 

Files

LValonMultiC-LZP.tif

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Additional details

Funding

European Commission
MechDeath – Study of the mechanical cues driving cell competition and its role in pretumoral cell expansion 789573
European Commission
CoSpaDD – Competition for Space in Development and Diseases 758457