G-quadruplex in the gene of the large subunit of plant RNA polymerase II: billion years old story

Supplementary material to the journal article:

Consist of:

• Difference CD spectra - comparison without and with previous UV treatment (Difference CD spectra - comparison without and with previous UV treatment.pdf)
• 40 bp long potential G4 locus is the most conserved site in the whole ~ 6000 bp long rpb1 gene. See the histogram below the alignment: the position of the G4 locus is depicted, together with the horizontal red dashed line indicating relative nucleotide conservation among aligned sequences of rpb1 (G4 locus is the most conserved within rpb1 gene.PNG)
• Prediction of G4-forming potential of inspected rpb1 locus(G4 sequences and scores.xlsx)
• Graphical abstract (Graphical abstract.PNG)
• PDB structure of modeled G-quadruplex structure (Modelled 3D structure of G4 from Bathycoccus prasinos.tar.gz)
• Multiple alignment of rpb1 paralogs (rpa1, rpc1, rpd1a, rpd1b) in Arabidopsis thaliana centered to G4 locus of rpb1 (Multiple_alignment_nrpsG4_arabidopsis.png)
• Multiple sequence alignment (using MUSCLE algorithm) of rpb1 CDS sequences (rpb1_aligned_sequences_all.txt)
• All 20 rpb1 CDS sequences in FASTA format (rpb1_sequences_all.txt)
• Five paralogs from Arabidopsis thaliana (rpa1,rpb1, rpc1, rpd1a, rpd1b) in FASTA format (rpbA_B_C_D sequences arabidopsis .txt)