Gradients2-MGL1704-FluorometricChlorophyll_2020_04-01_v1.0

Cruise: Gradients 2, MGL1704

Project Name: Simons Foundation, Gradients 2 NPSG

Description:

Fluorometric Chlorophyll were collected from rosette nisken bottles at specific depths and filtered at low vacuum through a glass fiber filter. Samples were then flash frozen in liquid nitrogen and stored in a histoprep capsule at -80°C until analysis. Post cruise, the filters were extracted using 90% acetone for a total of 48 hours in a -20°C freezer, covered to prevent the photodegradation of pigments. Upon extraction samples were centrifuged to settle filter particulates and gently poured into a glass cuvette. Samples were then run on a Turner 10-AU Fluorometer. Data were corrected for average glass fiber filter blank values and when available is reported as the average of duplicate samples (NaN indicates no duplicate sample).