Features of the in vitro propagation of Pseudotsuga menziesii (Mirb.) Franco

Modern propagation methods, one of which is in vitro, should be used to obtain the genetically valuable planting material of high quality. However, high costs and a total lack of equipment for effective propagation by this method significantly reduce the expected outcome percentage. In this case, the significance of the studied species propagation by this method involves a lack of influence of the periodicity of seed years; the possibility of plant propagation regardless of the time of year; the ability to choose in vitro planting material which has the characteristics being of interest to researchers; the obtaining of planting material that is genetically identical to maternal plant; the in vitro propagation using a small area; the propagation of explants without taking them out from the juvenile phase, etc (Dunstan et al. 1992, Ramage et al. 2002, Pullman et al. 2009).