Published February 1, 2021
| Version v2
Dataset
Open
Cofactor specificity of the tetrameric 6-phosphogluconate dehydrogenase from Gluconobacter oxydans: Studies of wild-type and mutants forms.
Authors/Creators
- 1. Laboratorio de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Chile
Description
Here we present the results regarding the characterization of the kinetics parameters for NAD and NADP of the 6-phosphogluconate dehydrogenase from Gluconobacter oxydans at different concentrations of 6PG.
The raw data of kinetic parameters for NAD or NADP at different 6PG concentrations are represented in kcat and KM, with the units s-1 and µM, respectively. Al the rest values as an efficient constant are represented in s-1µM-1.
Also, we included the kinetic characterizations of the mutants Go6PGDH-R31L, Go6PGDH-R31V, and Go6PGDH-D30N. The CSV files contain four columns. 1. NAD or NADP concentration in µM; 2. enzyme activity in U/mg; 3. Standard deviation and 4. The number of independent experiments.
Files
Cofactor preference vs 6PG.csv
Files
(3.0 kB)
| Name | Size | |
|---|---|---|
|
md5:01d999eef45a5bbaf917a36075c2a982
|
172 Bytes | Preview Download |
|
md5:b181738dc70c7f72919273c7132766b8
|
303 Bytes | Preview Download |
|
md5:a156659a16421e28b686d8ff3f5388e0
|
321 Bytes | Preview Download |
|
md5:c9e6b0096e360b88053f9ba66214d6b5
|
347 Bytes | Preview Download |
|
md5:bfde8b61ed00a48469d4148daf4af58d
|
329 Bytes | Preview Download |
|
md5:da246ce9245b3e9f032ab57e66099120
|
236 Bytes | Preview Download |
|
md5:f2cae1b041745f2c8c99bf5b3a1e1bb8
|
321 Bytes | Preview Download |
|
md5:62eb7ac5c51ea02fd62945812053beb1
|
276 Bytes | Preview Download |
|
md5:9053fb5eb659426e040b1c7b0ee534d4
|
371 Bytes | Preview Download |
|
md5:a3be5ccf8ca17e25d927f6727640397f
|
333 Bytes | Preview Download |