BioSense Institute - Research and Development Institute for Information Technologies in Biosystems
Published January 16, 2021 | Version v1
Journal article Open

Spectral-Phase Interferometry Detection of Ochratoxin A via Aptamer-Functionalized Graphene Coated Glass

Authors/Creators

  • 1. National Research University of Electronic Technology, 124498 Moscow, Russia;
  • 2. Moscow Institute of Physics and Technology, 9 Institutskii per., Dolgoprudny, 141700 Moscow, Russia; Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St, 119991 Moscow
  • 3. BioSense Institute-Research and Development Institute for Information Technologies in Biosystems, University of Novi Sad, 21000 Novi Sad, Serbia
  • 4. Graphenea, Avenida de Tolosa 76, 20018 Donostia-San Sebastián, Spain
  • 5. Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St, 119991 Moscow
  • 6. National Research University of Electronic Technology, 124498 Moscow, Russia; BioSense Institute-Research and Development Institute for Information Technologies in Biosystems, University of Novi Sad, 21000 Novi Sad, Serbia

Description

In this work, we report a novel method of label-free detection of small molecules based on direct observation of interferometric signal change in graphene-modified glasses. The interferometric sensor chips are fabricated via a conventional wet transfer method of CVD-grown graphene onto the glass coverslips, lowering the device cost and allowing for upscaling the sensor fabrication.For the first time, we report the use of graphene functionalized by the aptamer as the bioreceptor, in conjunction with Spectral-Phase Interferometry (SPI) for detection of ochratoxin A (OTA). In a direct assay with an OTA-specific aptamer, we demonstrated a quick and significant change of the optical signal in response to the maximum tolerable level of OTA concentration. The sensor regeneration is possible in urea solution. The developed platform enables a direct method of kinetic analysis of small molecules using a low-cost optical chip with a graphene-aptamer sensing layer.

Notes

This work was supported in part by the IPANEMA project, which received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement N° 872662, in part by the REALSENSE1: Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, Good Food Institute 2018 Competitive Grant Program, in part by the EU H2020 Graphene Flagship Core 3 Grant No. 881603, in part by the Russian Science Foundation under grant number 19-19-00401 (graphene chip development) and grant of the Ministry of Science and Higher Education of the Russian Federation for large scientific projects in priority areas of scientific and technological development (registration number 2020-1902-01-222, subsidy identifier 075-15-2020-774). I.G. and I.B. participated in a project that has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement N° 739570 (ANTARES).

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Additional details

Funding

European Commission
ANTARES - Centre of Excellence for Advanced Technologies in Sustainable Agriculture and Food Security 739570