AGO2 localises to cytokinetic protrusions in a p38 dependent manner and is needed for accurate cell division.
- Pantazopoulou, I. Vasiliki1
- Delis, D. Anastasios1
- Georgiou, Styliani1
- Pagakis, N. Stamatis2
- Filippa, Vasiliki1
- Dragona, Eleni1
- Kloukina, Ismini1
- Chatzitheodoridis, Elias3
- Trebicka, Jonel4
- Velentzas, D. Athanasios5
- Thiele, Maja6
- Gagos, Sarantis1
- Thanos, Dimitris1
- Tseleni-Balafouta, Sofia7
- Stravopodis, J. Dimitrios5
- Anastasiadou, Ema1
- 1. Department of Genetics, Center for Basic Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
- 2. Confocal Microscopy Laboratory, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
- 3. Division of Mineralogy, Petrology and Economic Geology, Department of Geological Sciences, School of Mining and Metallurgical Engineering, National Technical University of Athens, Athens, Greece
- 4. Division of Internal Medicine I, Department of Internal Medicine, University Clinic Bonn, Bonn, Germany
- 5. Section of Cell Biology and Biophysics, Department of Biology, School of Science, National and Kapodistrian University of Athens, Athens, Greece
- 6. Institute for Clinical Research, University of Southern Denmark, Odense, Denmark
- 7. Division of Pathology, Department of Pathology, Medical School, National and Kapodistrian University of Athens, Athens, Greece
Description
Argonaute 2 (AGO2) is an indispensable component of the RNA-induced silencing complex, operating at the transcriptional or posttranscriptional level. It is compartmentalized into structures such as GW- and P-bodies, stress granules and adherens junctions as well as the midbody. Here we show using immunofluorescence, image- and bioinformatic analysis and cytogenetics that AGO2 also resides in membrane protrusions such as open- and close-ended tubes. The latter are cytokinetic bridges where AGO2 colocalizes at the midbody-arms with cytoskeletal components such as α-Τubulin and Aurora B and various kinases. AGO2, phosphorylated on serine 387 is located together with Dicer at the midbody ring in a manner dependent on p38 MAPK activity. We further show that AGO2 is stress sensitive and important to ensure the proper chromosome segregation and cytokinetic fidelity. We suggest that AGO2 is part of a regulatory mechanism triggered by cytokinetic stress to generate the appropriate micro-environment for local transcript homeostasis.
Statement: AGO2 resides in open-ended tunneling nanotubes and close-ended cytokinetic bridges. At the latter location AGO2 colocalises with cell division components and the authors show that AGO2 deregulation impairs cell division fidelity.
Notes
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figure1.zip
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