Example Datasets for Iliski - Neuronal calcium, RBC velocities and fUS responses to odorant stimuli in the mouse olfactory bulb

Dataset containing 2 HDF5 files, one per mouse. It is intended to be used to test Iliski, a Transfer Function computation software. Iliski is available on GitLab (https://gitlab.com/AliK_A/iliski) along with the User Manual. Refer to the User Manual and to the ReadMe file for more details on Iliski. Data were already published on Zenodo (https://doi.org/10.5281/zenodo.3773863), but along an old version of the software. This upload is made for clarity purposes.

Each file contains acquisitions of responses to odorant stimuli in the olfactory bulb made with :

• two-photon linescan microscopy (for Ca2+ and RBC velocity);
• functional ultrafast ultrasound, acquired from a coronal plane.

HDF5 files tree is as follows :

• Data type
  • Raw : straight out of our extraction software, no specific treatment applied;
  • Aligned : every acquisition has been aligned so that the odor delivery matches the 10 s mark. Acquisitions have also been interpolated to be meaned;
  • Delta : aligned acquisitions are subtracted with the baseline value (between 5 and 10 s);
  • DetaOverBSL : aligned acquisitions are subtracted and then divided with the baseline value.
• Data source
  • Ca : calcium data from GCamP6f expressed in the mitral cells dendritic tufts;
  • RBC : RBC velocities in a capillary near the calcium recording site, simultaneously acquired;
  • FUS : fUS data, coronal plane. Only in FUS folder is two different folders then : High and Lowspeed, corresponding to different filter for fUS treatment,  > 80Hz and 10-30Hz respectively.
• Stimulation type : Odorant_Quantity_Duration
  • Odorant type, either Iso Amyl Acetate (AA) or Ethyl tiglate (ET);
  • Odor quantity : measured and calibrated in volt with a photo-ionizator;
  • Odor duration : from 5 s down to 120 ms, a single sniff for a mouse.

Ca2+ : Calcium

fUS : functional ultrafast ultrasound

RBC : Red Blood Cell