Antibacterial properties and phytochemical screening of Tamarix nilotica leaves from Sudan
Authors/Creators
- 1. Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, Sudan.
- 2. Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, Sudan.Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, Sudan.
- 3. Department of Biotechnology, Faculty of Science and Technology, Omdurman Islamic University, Sudan.
- 4. Department of Microbiology, Faculty of Medical Laboratories, Omdurman Islamic University, Sudan.
- 5. Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, Sudan.Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, Sudan.Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, Sudan.
Description
The antibacterial activity of crude extracts of leaves coated with wax and other de-waxed leaves of Tamarix nilotica, against nine human pathogenic bacteria were evaluated using the agar plate well-diffusion method with plant extract concentration of 100 mg/ml. The minimum inhibitory concentration (MIC) was determined. The antibacterial activity was found in the least MIC, as 7.5 mg/ml, belonged to Staphylococcus aureus and Pseudomonas aeruginosa. Most susceptible gram-negative bacteria were P. aeruginosa and least susceptible gram-negative bacteria were E. coli and S. typha. In gram-positive bacteria, most and least susceptible were S. aureus and B. cereus, respectively. These findings should stimulate the research for novel, natural product such as many anti-bacterial agents. The percentage yield of epicuticle wax from Tamarix nilotica leaves, extracted with n-hexane was 0.7%. The results of the preliminary phytochemical analyses of the ethanol extract of the leaves coated with wax and de-waxed leaves of Tamarix nilotica were performed. Steroids, phenolic compounds, saponins, tannins, flavonoids and sugars are present in all of the two samples analyzed except sugars in de-waxed leaves.