Published August 28, 2020 | Version v2

De novo genome assembly of the Tobacco Hornworm moth (Manduca sexta)

  • 1. Johns Hopkins University Department of Molecular Biology and Genetics
  • 2. Johns Hopkins University Department of Biomedical Engineering
  • 3. Northeastern University, Department of Biology, Boston, MA

Description

We present the new reference genome for M sexta, JHU_Msex_v1.0, applying a combination of modern technologies in a de novo assembly to increase continuity, accuracy, and completeness. The assembly is 470 Mb and is ~25x more continuous than the original assembly, with scaffold N50 >14 Mb. We annotated the assembly by lifting over existing annotations and supplementing with additional supporting RNA-based data for a total of 25,256 genes. The new reference assembly is accessible in annotated form for public use.

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Supplemental_material.pdf

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